Anti-Caspase-1 antibody (ab62698)
Key features and details
- Rabbit polyclonal to Caspase-1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Caspase-1 antibody
See all Caspase-1 primary antibodies -
Description
Rabbit polyclonal to Caspase-1 -
Host species
Rabbit -
Specificity
ab62698 recognizes Caspase-1 subunit p10. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Caspase-1.
Database link: P29466 -
Positive control
- Human brain tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
ab62698 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62698 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000.
|
|
IHC-P |
1/50 - 1/100.
|
Notes |
---|
WB
1/500 - 1/1000. |
IHC-P
1/50 - 1/100. |
Target
-
Function
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. -
Tissue specificity
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 834 Human
- Omim: 147678 Human
- SwissProt: P29466 Human
- Unigene: 2490 Human
-
Alternative names
- CASP-1 antibody
- CASP1 antibody
- CASP1_HUMAN antibody
see all
Images
-
ab62698 at a 1:50 dilution, staining Caspase-1 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.
-
All lanes : Anti-Caspase-1 antibody (ab62698)
Lane 1 : 293 cell extract
Lane 2 : MCF-7 cell extract
Lane 3 : HeLa cell extract
Lane 4 : 293 cell extract with blocking peptide
Observed band size: 29 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (20)
ab62698 has been referenced in 20 publications.
- Pu J et al. Immunohistochemical analysis of pyroptosis-related protein expression in IgG4-related sialadenitis. J Oral Pathol Med 51:388-394 (2022). PubMed: 35218237
- Zha S et al. Topical Simvastatin Improves Lesions of Diffuse Normolipemic Plane Xanthoma by Inhibiting Foam Cell Pyroptosis. Front Immunol 13:865704 (2022). PubMed: 35619689
- Schweizer TA et al. Intervertebral disc cell chondroptosis elicits neutrophil response in Staphylococcus aureus spondylodiscitis. Front Immunol 13:908211 (2022). PubMed: 35967370
- Zhang M et al. miR-181a-5p Inhibits Pyroptosis in Sepsis-Induced Acute Kidney Injury through Downregulation of NEK7. J Immunol Res 2022:1825490 (2022). PubMed: 35991122
- Su Q et al. Antituberculosis Drugs (Rifampicin and Isoniazid) Induce Liver Injury by Regulating NLRP3 Inflammasomes. Mediators Inflamm 2021:8086253 (2021). PubMed: 33688304