Recombinant
RabMAb

Anti-Caspase-1 antibody [EPR19672] (ab207802)

Overview

  • Product name
    Anti-Caspase-1 antibody [EPR19672]
    See all Caspase-1 primary antibodies
  • Description
    Rabbit monoclonal [EPR19672] to Caspase-1
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Caspase-1 aa 100-300. The exact sequence is proprietary.
    Database link: P29466

  • Positive control
    • WB: Untreated THP-1 whole cell lysate; THP-1 treated with 80 nM TPA overnight, then treated with 1 µg/ml LPS for 8 hours whole cell lysate; Human lymph node, thymus and spleen lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab207802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/1000. Detects a band of approximately 45, 42, 35, 22, 20 kDa (predicted molecular weight: 45, 42, 35, 22, 20 kDa).

Target

  • Function
    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • Tissue specificity
    Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CASP-1 antibody
    • CASP1 antibody
    • CASP1_HUMAN antibody
    • Caspase 1 antibody
    • Caspase-1 subunit p10 antibody
    • ICE antibody
    • IL-1 beta-converting enzyme antibody
    • IL-1BC antibody
    • IL1 beta converting enzyme antibody
    • IL1B convertase antibody
    • Interleukin 1 beta convertase antibody
    • Interleukin 1B converting enzyme antibody
    • Interleukin-1 beta convertase antibody
    • Interleukin-1 beta-converting enzyme antibody
    • p45 antibody
    see all

Images

  • All lanes : Anti-Caspase-1 antibody [EPR19672] (ab207802) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 2 : THP-1 (Human monocytic leukemia cell line) treated with 80 nM TPA overnight, then treated with 1 µg/ml LPS for 8 hours whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 45, 42, 35, 22, 20 kDa
    Observed band size : 20,22,42,45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

    There are six pro-caspase 1 isoforms, generating products between 30-45kDa, Pro-Caspase 1 is cleaved into p20(p22) and p10(p12) subunits. PMID: 8662843. PMID: 7595215. The product recognizes p20(p22) cleavage fragment where the immunogen is located.

  • All lanes : Anti-Caspase-1 antibody [EPR19672] (ab207802) at 1/1000 dilution

    Lane 1 : Human lymph node lysate
    Lane 2 : Human thymus lysate
    Lane 3 : Human spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size : 45, 42, 35, 22, 20 kDa
    Observed band size : 22,35,45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Pro-Caspase 1 is cleaved into p20(p22) and p10(p12) subunits. PMID: 8662843. PMID: 7595215. The product recognizes p20(p22) cleavage fragment where the immunogen is located.

  • ab207802 Immunoprecipitating Caspase-1 in THP-1 (human acute monocytic leukemia) whole cell lysate. 2µg of capture antibody in 0.35mg lysate was used. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/1000 and VeriBlot for IP secondary antibody (HRP) ab131366 was used as the secondary antibody at a dilution of 1/1000.

    Lane 1: THP-1 (human acute monocytic leukemia ) whole cell lysate
    Lane 2: THP-1 (human acute monocytic leukemia ) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207802 in THP-1 (human acute monocytic leukemia ) whole cell lysate

    Blocking buffer: 5% NFDM/TBST

    Diluting buffer: 5% NFDM/TBST

References

ab207802 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application
Western blot
Sample
Human Cell lysate - whole cell (LX-2 Human Hepatic Stellate Cell Line)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
LX-2 Human Hepatic Stellate Cell Line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 14 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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