The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 45, 42, 35, 22, 20 kDa (predicted molecular weight: 45, 42, 35, 22, 20 kDa).
Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
Belongs to the peptidase C14A family. Contains 1 CARD domain.
The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
There are six pro-caspase 1 isoforms, generating products between 30-45kDa, Pro-Caspase 1 is cleaved into p20(p22) and p10(p12) subunits. PMID: 8662843. PMID: 7595215. The product recognizes p20(p22) cleavage fragment where the immunogen is located.
Western blot - Anti-Caspase-1 antibody [EPR19672] (ab207802)
All lanes : Anti-Caspase-1 antibody [EPR19672] (ab207802) at 1/1000 dilution
Lane 1 : Human lymph node lysate Lane 2 : Human thymus lysate Lane 3 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
ab207802 Immunoprecipitating Caspase-1 in THP-1 (human acute monocytic leukemia) whole cell lysate. 2µg of capture antibody in 0.35mg lysate was used. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/1000 and VeriBlot for IP secondary antibody (HRP) ab131366 was used as the secondary antibody at a dilution of 1/1000.
Lane 1: THP-1 (human acute monocytic leukemia ) whole cell lysate Lane 2: THP-1 (human acute monocytic leukemia ) whole cell lysate Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207802 in THP-1 (human acute monocytic leukemia ) whole cell lysate