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Overview

  • Product nameCaspase 1 Assay Kit (Colorimetric)See all Caspase 1 kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Caspase 1 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate YVAD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of p-NA from a treated sample with an untreated control allows determination of the fold increase in Caspase 1 activity.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCaspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role in down stream of Caspase 8 which is involved in Fas-mediated apoptosis.
  • Cellular localizationCytoplasmic
    • Alternative names
        CASP 1CASP 1CASP1
        Caspase 1 apoptosis related cysteine peptidaseCaspase 1 apoptosis related cysteine proteaseCaspase 1 apoptosis related cysteine proteaseCaspase1ICEIL 1 beta converting enzymeIL 1 beta converting enzymeIL 1BCIL1B convertaseIL1B convertaseIL1BCIL1BCEInterleukin 1 beta convertaseInterleukin 1 beta convertase precursorInterleukin 1 beta convertase precursorInterleukin 1 beta converting enzymeInterleukin 1B converting enzymeInterleukin 1B converting enzymeP45
      see all

    Applications

    Our Abpromise guarantee covers the use of ab39470 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Protocols

    References for Caspase 1 Assay Kit (Colorimetric) (ab39470)

    This product has been referenced in:
    • Stout-Delgado HW  et al. Impaired NLRP3 inflammasome function in elderly mice during influenza infection is rescued by treatment with nigericin. J Immunol 188:2815-24 (2012). Mouse . Read more (PubMed: 22327078) »

    See 1 Publication for this product

    Publishing research using ab39470 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab39470 Submit a Question for ab39470

    Displaying 1 - 10 of 16 results for Abreviews and Q&A

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    I had a question on sample prep for the caspase-1 assay kit (ab39470). I am planning on treating cells (that are adherent) in a six well dish. What is the best way to prep these samples for this assay? I am a little lost on how the cell lysis buffer shoul...

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    Basically, you want to have 100 - 200 ug of protein in a 50 ul volume for each sample. If you are starting with unlysed cells, follow the instructions and lyse them with the provided lysis buffer, and adjust the protein concentration to 100 - 200 ug per 5...

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    Customer kindly contacted us to inquire if this product would work on previously lysed with a RIPA buffer.

    Yes you can use lysates made with RIPA buffer. Use ~100-200 µg/assay for efficient results.

    Dear Supplier,
    Please see customers request regarding, Caspase 1 Assay Kit (Colorimetric) (ab39470):
    We would like to measure Caspase-1 bioactivity in lysates of murine splenocytes and I am particularly interested in the kit from Abcam (#ab39470...

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    Thank you for contacting us.

    This kit has been tested in house with a human cell line (Jurkat cells), but it should also work for almost all mammalian cells. We have several reports from other customers who have used this kit with mouse samples s...

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    Is the 2X reaction buffer available as a separate item? We are using it up for our controls.

    Thank you for contacting us. I apologize for the delay in getting the Capase 1 assay 2X buffer into our catalogue as a separate item. It is available as ab135438:

    Click here (or use the following: http://www.abcam.com/index.html?datasheet=13543...

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    I have purchased the ab39470 kit however the 2x reaction buffer has run out. Is this buffer available for purchase on its own? If not, is it possible for find out the formula?

    Regards

    Thank you for contacting us.

    We can not provide the formula of reaction buffer we however can provide the buffer as a special request. The cost would be GBP 75 plus shipping charges. Please let me know if you are interested in buying this product...

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    Can the kit be used with tissue that has been frozen but not lysed? Is there a protocol for using the kit with tissue (the protocol is written for cell lysates)?

    Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiries.

    The lab has only tested this kit with fresh samples, so we do not have any data from using the kit with frozen tissue. When...

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    How should the protein concentration be measured? The lysis buffer is interfering with a Coomassie assay.

    Thank you for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

    The lab uses either a BCA or a Coomassie assay kit to measure the protein concentration of the lysates.

    Here are so...

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    Is this kit in fact specific for caspase 1, or can other caspases cleave this substrate?

    Thank you for contacting us. The developer of the kit thinks that the substrate is for caspase-1/ICE and related caspases that recognize the amino acid sequence YVAD, and that " it is not exclusive to Caspase 1."

    However, he did not provide any...

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    I am sorry to trouble you. I would like to buy two Elisa-Kit to detect 1.mature mouse IL-1β and 2.mature mouse caspase-1 ，and I don't want it have a corssreactivity with pro-IL-1β and pro- caspase-1 , I found your company have these kit, could...

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    Thank you for contacting Abcam.

    For ab39470, it measures the mature form of Caspase-1 and for ab100705, IL1 beta Mouse ELISA Kit - this kit will recognize the mature form (aa 118-269) of IL1beta. Since the pro IL1beta also contains this amino ac...

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    Phone call requesting information on whether mouse, human or both samples can be used with this kit.

    Thank you for contacting us and your interest in our products.


    The Caspase 1 Colorimetric Assay Kit has been tested in house with a human cell line (Jurkat cells), but the kitshould work for almost all mammalian cells. We have several repo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"