Caspase 1 Inhibitor Drug Detection Kit (ab102489)

Overview

  • Product nameCaspase 1 Inhibitor Drug Detection Kit
    See all Caspase 1 kits
  • Detection methodFluorescent
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Assay time
    1h 30m
  • Product overview

    Abcam's Caspase 1 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate YVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-1 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-1 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
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  • Notes

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • Tested applicationsSuitable for: Functional Studiesmore details
  • PlatformMicroplate

Properties

  • RelevanceCaspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role in down stream of Caspase 8 which is involved in Fas-mediated apoptosis.
  • Cellular localizationCytoplasmic
  • Alternative names
    • CASP1
    • CASP1 nirs variant 1
    • Caspase 1 apoptosis related cysteine peptidase
    • caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
    • ICE
    • ICE-like protease
    • IL1 B convertase
    • IL1 beta converting enzyme
    • IL1BC
    • IL1BCE
    • Interleukin 1 B converting enzyme
    • Interleukin 1 beta convertase
    • P45
    see all

Applications

Our Abpromise guarantee covers the use of ab102489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Caspase 1 Inhibitor Drug Detection Kit images

  • Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using YVAD-AFC as caspase 1 substrate and following Caspase 1 Inhibitor Drug Detection Kit (ab102489) protocol. Lanes:

    1.- Background Control: no Caspase 1, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 1, +z-VAD-FMK

    3.- Positive Control: + Caspase 1, no z-VAD-FMK

    4.- Caspase 1 + 2.5µM z-VAD-FMK

    5.- Caspase 1 + 10µM z-VAD-FMK

    6.- Caspase 1 + 40µM z-VAD-FMK

Protocols

References for Caspase 1 Inhibitor Drug Detection Kit (ab102489)

ab102489 has not yet been referenced specifically in any publications.

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I can confirm this kit is designed to screen caspase-1 inhibitors. This can be done in 2 ways:

Putative caspase-1 inhibitors used to treat cells expressing caspase-1 can be compared for the inhibi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"