Anti-Caspase-1 (phospho S376) antibody (ab61794)

Overview

  • Product nameAnti-Caspase-1 (phospho S376) antibody
    See all Caspase-1 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-1 (phospho S376)
  • Specificityab61794 detects endogenous levels of Caspase-1 only when phosphorylated at serine 376.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-1.
    Database link: P29466

  • Positive control
    • Human brain tissue and 293 cell extracts.
  • General notes

    The amino acid range of this immunogen is aa350-400 of human Caspase-1 with Acc# P29466 (http://www.uniprot.org/uniprot/P29466). This antibody binds to the Caspase-1 subunit p10 region. It binds to isoforms alpha, beta, gamma, delta, and epsilon.

Properties

Applications

Our Abpromise guarantee covers the use of ab61794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 45 kDa).

Target

  • FunctionThiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
  • Tissue specificityExpressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CASP-1 antibody
    • CASP1 antibody
    • CASP1_HUMAN antibody
    • Caspase 1 antibody
    • Caspase-1 subunit p10 antibody
    • ICE antibody
    • IL-1 beta-converting enzyme antibody
    • IL-1BC antibody
    • IL1 beta converting enzyme antibody
    • IL1B convertase antibody
    • Interleukin 1 beta convertase antibody
    • Interleukin 1B converting enzyme antibody
    • Interleukin-1 beta convertase antibody
    • Interleukin-1 beta-converting enzyme antibody
    • p45 antibody
    see all

Anti-Caspase-1 (phospho S376) antibody images

  • All lanes : Anti-Caspase-1 (phospho S376) antibody (ab61794) at 1/500 dilution

    Lane 1 : 293 cell extracts
    Lane 2 : 293 cell extracts with immunising peptide


    Predicted band size : 45 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab61794 at 1:50 - 1:100 dilution staining Caspase-1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

References for Anti-Caspase-1 (phospho S376) antibody (ab61794)

ab61794 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.

We do not have any experimental data regarding the reactivity of this antibody to the active or inactive form of Caspase 1. By sequence homology this antibody will recognize both the inactive and active forms of Caspa...

Read More

Thank you for contacting Abcam.
Based on the information from UniPort (SwissProt: P29466), there are a number of isoforms of Caspase-1, one of which is about 30kDa. I am not sure which isoforms is expressed in 293 cell extracts.
Although we d...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"