Overview

  • Product nameAnti-Caspase-10 antibody
    See all Caspase-10 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-10
  • SpecificityThis antibody only recognizes FLICE2 form of caspase-10.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-10 aa 505-521 (C terminal).
    Sequence:

    ISAQTPRPPMRRWSSVS


    Database link: Q92851
    (Peptide available as ab8377)

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).Can be blocked with Human Caspase-10 peptide (ab8377).
ICC/IF 1/50. Fix with PFA.

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
    -AMC and Asp-Glu-Val-Asp-
    -AMC.
    Isoform C is proteolytically inactive.
  • Tissue specificityDetectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
  • Involvement in diseaseDefects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
    Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
    Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • Post-translational
    modifications
    Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPS2 antibody
    • Apoptotic protease Mch-4 antibody
    • CASP 10 antibody
    • CASP-10 antibody
    • CASP10 antibody
    • CASPA_HUMAN antibody
    • Caspase 10 apoptosis related cysteine peptidase antibody
    • Caspase-10 subunit p12 antibody
    • FADD like ICE2 antibody
    • Fas associated death domain protein antibody
    • FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody
    • FLICE 2 antibody
    • FLICE2 antibody
    • ICE like apoptotic protease 4 antibody
    • ICE-like apoptotic protease 4 antibody
    • Interleukin 1B converting enzyme 2 antibody
    • MCH 4 antibody
    see all

Anti-Caspase-10 antibody images

  • All lanes : Anti-Caspase-10 antibody (ab2012) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Jurkat whole cell lysate
    Lane 3 : A431 whole cell lysate
    Lane 4 : K562 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate


    Predicted band size : 59 kDa
    Observed band size : 59 kDa
  • ab2012 at 1:50 staining cultured mouse hippocampal astrocytes by ICC/IF. The astrocytes were maintained in culture for 2 days in the presence of 10% serum, fixed with 4% PFA in PBS for 15 min at room temperature and permeabilized for 5 min in 0.25% Triton X-100 in PBS. The tissue was incuabated with the antibody for 2 hours at room temperature. A Cy3 ® conjugated donkey anti-rabbit IgG (H+L) was used as the secondary.

    See Abreview

References for Anti-Caspase-10 antibody (ab2012)

This product has been referenced in:
  • Pagnan G  et al. The combined therapeutic effects of bortezomib and fenretinide on neuroblastoma cells involve endoplasmic reticulum stress response. Clin Cancer Res 15:1199-209 (2009). WB ; Human . Read more (PubMed: 19228726) »
  • Green KA  et al. Caspase-mediated Cleavage of Insulin Receptor Substrate. J Biol Chem 279:25149-56 (2004). Read more (PubMed: 15069074) »

See all 2 Publications for this product

Product Wall

Thank you for your interest in our products. Although it has been years since the last enquiry, this product has yet to be tested in immunohistochemistry. However, we have just bought a new facility in the UK, equipped with high tech machines, that...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (cultured hippocampal astrocytes)
Specification cultured hippocampal astrocytes
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

Submitted Mar 08 2007

We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody ...

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As far as we are aware, Rabbit polyclonal to Caspase 10 (C-term) (ab2012), has not been tested in IHC.

Yes, anti-caspase-10 is mouse reactive. 3T3 cell lysate was tested by Western blot. $195 /100 µg + $25 for FedEx overnight shipping.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"