Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp- -AMC and Asp-Glu-Val-Asp- -AMC. Isoform C is proteolytically inactive.
Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
Involvement in disease
Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects. Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss. Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
Belongs to the peptidase C14A family. Contains 2 DED (death effector) domains.
Cleavage by granzyme B and autocatalytic activity generate the two active subunits. Phosphorylated upon DNA damage, probably by ATM or ATR.
Immunocytochemistry/Immunofluorescence of HeLa cells labeling Caspase-10 with Anti-Caspase-10 antibody (ab2012) at 5μg/ml.
Western blot - Anti-Caspase-10 antibody (ab2012)
All lanes : Anti-Caspase-10 antibody (ab2012) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate Lane 2 : Jurkat whole cell lysate Lane 3 : A431 whole cell lysate Lane 4 : K562 whole cell lysate Lane 5 : NIH3T3 whole cell lysate
Predicted band size: 59 kDa Observed band size: 59 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-10 antibody (ab2012)This image is courtesy of an anonymous Abreview
ab2012 at 1:50 staining cultured mouse hippocampal astrocytes by ICC/IF. The astrocytes were maintained in culture for 2 days in the presence of 10% serum, fixed with 4% PFA in PBS for 15 min at room temperature and permeabilized for 5 min in 0.25% Triton X-100 in PBS. The tissue was incuabated with the antibody for 2 hours at room temperature. A Cy3 ® conjugated donkey anti-rabbit IgG (H+L) was used as the secondary.
Pagnan G et al. The combined therapeutic effects of bortezomib and fenretinide on neuroblastoma cells involve endoplasmic reticulum stress response. Clin Cancer Res15:1199-209 (2009).
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