Overview

  • Product nameAnti-Caspase-10 antibody
    See all Caspase-10 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-10
  • Specificityab52225 detects endogenous levels of total Caspase-10 protein.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-10.
    Database link: Q92851

  • Positive control
    • IHC-P: human lung carcinoma WB: extracts from HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab52225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/1000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
    -AMC and Asp-Glu-Val-Asp-
    -AMC.
    Isoform C is proteolytically inactive.
  • Tissue specificityDetectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
  • Involvement in diseaseDefects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
    Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
    Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • Post-translational
    modifications
    Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPS2 antibody
    • Apoptotic protease Mch-4 antibody
    • CASP 10 antibody
    • CASP-10 antibody
    • CASP10 antibody
    • CASPA_HUMAN antibody
    • Caspase 10 apoptosis related cysteine peptidase antibody
    • Caspase-10 subunit p12 antibody
    • FADD like ICE2 antibody
    • Fas associated death domain protein antibody
    • FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody
    • FLICE 2 antibody
    • FLICE2 antibody
    • ICE like apoptotic protease 4 antibody
    • ICE-like apoptotic protease 4 antibody
    • Interleukin 1B converting enzyme 2 antibody
    • MCH 4 antibody
    see all

Anti-Caspase-10 antibody images

  • All lanes : Anti-Caspase-10 antibody (ab52225) at 1/300 dilution

    Lane 1 : extracts from HeLa cells,
    Lane 2 : extracts from HeLa cells, with immunizing peptide


    Predicted band size : 59 kDa
    Observed band size : 59 kDa
  • Immunohistochemical analysis of Caspase-10 expression in paraffin embedded human lung carcinoma tissue, using ab52225 at a dilution of 1:50.
    Left: untreated sample. Right: sample treated with immunising peptide.

    Immunohistochemical analysis of Caspase 10 expression in paraffin embedded human lung carcinoma tissue, using ab52225 at a dilution of 1/50.
    Left: untreated sample. Right: sample treated with immunising peptide.

References for Anti-Caspase-10 antibody (ab52225)

ab52225 has not yet been referenced specifically in any publications.

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