• Product nameAnti-Caspase-10 antibody
    See all Caspase-10 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-10
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-10 aa 100-200 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q92851
    (Peptide available as ab101384)

  • Positive control
    • This antibody gave a positive signal in Human kidney and lung tissue lysates as well as HeLa and A431 whole cell lysates.



Our Abpromise guarantee covers the use of ab92844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).


  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
    -AMC and Asp-Glu-Val-Asp-
    Isoform C is proteolytically inactive.
  • Tissue specificityDetectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.
  • Involvement in diseaseDefects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
    Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
    Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • Post-translational
    Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPS2 antibody
    • Apoptotic protease Mch-4 antibody
    • CASP 10 antibody
    • CASP-10 antibody
    • CASP10 antibody
    • CASPA_HUMAN antibody
    • Caspase 10 apoptosis related cysteine peptidase antibody
    • Caspase-10 subunit p12 antibody
    • FADD like ICE2 antibody
    • Fas associated death domain protein antibody
    • FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody
    • FLICE 2 antibody
    • FLICE2 antibody
    • ICE like apoptotic protease 4 antibody
    • ICE-like apoptotic protease 4 antibody
    • Interleukin 1B converting enzyme 2 antibody
    • MCH 4 antibody
    see all

Anti-Caspase-10 antibody images

  • All lanes : Anti-Caspase-10 antibody (ab92844) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Lung (Human) Tissue Lysate
    Lane 4 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 59 kDa
    Additional bands at : 53 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-Caspase-10 antibody (ab92844)

ab92844 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS

Dr. Kirk McManus

Verified customer

Submitted Jun 01 2012