Overview

  • Product nameAnti-Caspase-11 antibody
    See all Caspase-11 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-11
  • Specificityab22684 recognises caspase-11 proenzyme (43 kD) and the large (23 kD) and small (10 kD) subunits of the mature enzyme. No crossreactivity with other caspases tested: mouse caspases-1 and 3, human caspases 1 to 10 and 14.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    Recombinant full length protein corresponding to Mouse Caspase-11.
    Database link: P70343

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/15000. Predicted molecular weight: 43, 23 , 10 kDa.

Target

  • FunctionInvolved in ER-stress induced apoptosis. Cleaves caspase-1 (By similarity). Involved in the activation cascade of caspases responsible for apoptosis execution. Promotes IL-1 beta processing by ICE, so may also have a role in inflammatory responses.
  • Tissue specificityMostly expressed in lung and spleen; weaker in heart and lung; little in liver, skeletal muscle, kidney and testis. Not found in the brain.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The two subunits are derived from the precursor sequence by a autocatalytic mechanism.
  • Cellular localizationEndoplasmic reticulum membrane. Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • CASP-11 antibody
    • CASP-4 antibody
    • CASP11 antibody
    • CASP4 antibody
    • CASP4_MOUSE antibody
    • Caspase 11 antibody
    • Caspase 11 apoptosis related cysteine peptidase antibody
    • Caspase 11 apoptosis related cysteine protease antibody
    • Caspase 4 antibody
    • Caspase 4 apoptosis related cysteine peptidase antibody
    • Caspase-11 antibody
    • Caspase-4 subunit p20 antibody
    • CASPL antibody
    • Ich 3 antibody
    • Ich3 antibody
    • ICH3 protease antibody
    • Protease ICH-3 antibody
    see all

Anti-Caspase-11 antibody images

  • All lanes : Anti-Caspase-11 antibody (ab22684) at 1/10000 dilution

    Lane 1 : Mouse liver whole cell lysate
    Lane 2 : Mouse hepatocyte whole cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43, 23 , 10 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Caspase-11 antibody (ab22684)

This product has been referenced in:
  • Xu J  et al. Macrophage endocytosis of high-mobility group box 1 triggers pyroptosis. Cell Death Differ N/A:N/A (2014). Read more (PubMed: 24769733) »
  • Kang MJ  et al. IL-18 is induced and IL-18 receptor alpha plays a critical role in the pathogenesis of cigarette smoke-induced pulmonary emphysema and inflammation. J Immunol 178:1948-59 (2007). WB ; Mouse . Read more (PubMed: 17237446) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver, hepatocytes)
Specification liver, hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 31 2014

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (12% gel)
Sample Mouse Cell lysate - whole cell (Bone Marrow Derived Macrophages)
Specification Bone Marrow Derived Macrophages
Treatment LPS 100ng/mL for 5hr
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Ms. Connie Slocum

Verified customer

Submitted Sep 13 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"