Overview

  • Product nameCaspase 12 (active) FITC Staining Kit
    See all Caspase 12 kits
  • Assay typeEnzyme activity
  • Product overview

    The Caspase 12 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase 12 in living cells. The assay utilizes the caspase 12 inhibitor, ATAD-FMK, conjugated to FITC (FITC-ATAD-FMK) as a marker. FITC-ATAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase 12 in apoptotic cells. The FITC label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.

  • Tested applicationsSuitable for: Functional Studies, Flow Cytmore details

Properties

  • RelevanceCaspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. Caspase 12 is most highly related to members of the ICE subfamily. However, human caspase 12 has no protease activity. It may reduce cytokine release in response to bacterial lipopolysaccharide during infections and reduces activation of NF-kappa-B in response to TNF.
  • Alternative names
    • CASP12
    • CASP12P1
    • Caspase 12
    • Inactive caspase 12

Associated products

Applications

Our Abpromise guarantee covers the use of ab65610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

Protocols

References for Caspase 12 (active) FITC Staining Kit (ab65610)

This product has been referenced in:
  • Ryan SD  et al. Neuronal dystonin isoform 2 is a mediator of endoplasmic reticulum structure and function. Mol Biol Cell 23:553-66 (2012). Read more (PubMed: 22190742) »

See 1 Publication for this product

Product Wall

The developer of the kit suggests trypsinizing the cells and then using the protocol for cells in suspension. If you do the assay this way, it would probably be best any apply the apoptosis-inducing treatment to the cells after trypsinization, to the c...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"