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The Caspase 12 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase 12 in living cells. The assay utilizes the caspase 12 inhibitor, ATAD-FMK, conjugated to FITC (FITC-ATAD-FMK) as a marker. FITC-ATAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase 12 in apoptotic cells. The FITC label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
|Components||25 tests||100 tests|
|FITC-ATAD-FMK||1 x 25µl||1 x 100µl|
|Wash Buffer||1 x 50ml||2 x 100ml|
|Z-VAD-FMK||1 x 10µl||1 x 10µl|
Our Abpromise guarantee covers the use of ab65610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
|Flow Cyt||Use at an assay dependent dilution.|