Overview

  • Product nameAnti-Caspase-12 antibody [14F7]
    See all Caspase-12 primary antibodies
  • Description
    Rat monoclonal [14F7] to Caspase-12
  • SpecificityThis antibody recognizes the full length and possibly the truncated p20 subunit of Caspase-12
  • Tested applicationsSuitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Mouse Caspase-12 aa 95-318.
    Sequence:

    GPQKICTPSS PSESKRKVEDD EMEVNAGLAH ESHLM LTAPH GLQSSEVQDTL KLCPRDQFCK IKTERAKEIY PVMEKEGRTRL ALIICNKKFDY LFDRDNADTDI LNMQELLENLG YSVVLKENLTA QEMETELMQFA GRPEHQSSDST FLVFMSHGIL EGICGVKHRNK KPDVLHDDTIF KIFNNSNCRSL RNKPKILIMQA CRGRYNGTIWV STNKGIATAD


    Database link: Q6UXS9

  • Positive control
    • A whole cell extract of human hepatocellular carcinoma HepG2 cells.
  • General notes

    For continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab10455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.

Determined by using a whole cell extract of human hepatocellular carcinoma HepG2 cells

ICC Use at an assay dependent concentration.

4% paraformaldehyde-fixed, paraffin-embedded

Target

References for Anti-Caspase-12 antibody [14F7] (ab10455)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for your enquiry. Ab10455 recognizes the full length and possibly the truncated p20 subunit of Caspase 12. If you have any additional questions, please contact us again.

The source of ab10455 told us: "The antibody is produced by fusion with mouse myeloma. According to Antibodies: A Laboratory Manual by Harlow and Lane, mouse myelomas take on the characteristics of the cells its fused to. Thus, if a mouse myeloma w...

Read More

Thank you very much for your enquiry. For both ab8117 and ab10455, the band should be at about 50-55 kDa. If you have any further questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"