• Product nameAnti-Caspase-12 antibody
    See all Caspase-12 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-12
  • SpecificityRecognises full-length and cleaved fragment of Caspase-12
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-12.
    Database link: Q6UXS9
    (Peptide available as ab38274)

  • Positive control
    • Spleen tissue lysate



Our Abpromise guarantee covers the use of ab18766 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 4 µg/ml. Detects procaspase 12 (55 kDa) and cleavage caspase 12 (36 kDa).
ICC/IF Use a concentration of 1 µg/ml.


Anti-Caspase-12 antibody images

  • Anti-Caspase-12 antibody (ab18766) + Mouse spleen tissue lysate
  • Anti-Caspase-12 antibody (ab18766) + Rat kidney tissue lysate
  • ab18766 (1µg/ml) staining caspase-12 in human liver using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm and nuclei in hepatic arteries and plates of hepatic cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • ICC/IF image of ab18766 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18766, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1:1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Caspase-12 antibody (ab18766)

This product has been referenced in:
  • Crespo I  et al. Glutamine Treatment Attenuates Endoplasmic Reticulum Stress and Apoptosis in TNBS-Induced Colitis. PLoS One 7:e50407 (2012). WB ; Rat . Read more (PubMed: 23209735) »
  • Yang X  et al. Neurodegenerative and Inflammatory Pathway Components Linked to TNF-a/TNFR1 Signaling in the Glaucomatous Human Retina. Invest Ophthalmol Vis Sci 52:8442-54 (2011). WB ; Human . Read more (PubMed: 21917936) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry. Unfortunately, the exact immunogen sequence for the ab38276 peptide is proprietary and can not be released. The supplier was kind enough to inform us that the sequence is located around the 310 a.a. however any further i...

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I sincerely apologize for the wait. I finally manage to get some confirmation from the originator of the product regarding the immunogen sequence. I can confirm that the immunogen sequence for this ab18766 is located near the 310 amino acid, and th...

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Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab18766 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the ...

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Thank you for your enquiry. I can confirm that this product will recognize the full-length and cleaved fragment of Caspase 12. Unfortunately, we do not have any info on whether this product can also detect the pro caspase 12 form. In order to detec...

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Thank you for your enquiry. The antibody can detect ~50 kDa unprocessed protein, and also the cleaved fragment at around 30-40 kDa. You may not see the unprocessed precursor protein in all samples, since the protein will be processed and cleaved be...

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Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you d...

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Thank you for your email. Unfortunately we do not have a Western blot image available at this time. Please rest assured that we guarantee that ab18766 will work in Western blotting. Our policy at Abcam is that if an antibody does not work as specif...

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Thank you for your enquiry. Ab18766 recognizes full-length and cleaved fragment of Caspase 12. We are currently not aware of any publications featuring the use of this antibody. If you have any additional questions, please contact us again.