Customer reviews and FAQs

Overview

  • Product name
    Anti-Caspase-12 antibody
    See all Caspase-12 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-12
  • Specificity
    This antibody reacts with both the propeptide and cleaved forms of Caspase-12.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-12. immunogen sequence from within aa 181 to 240
    Database link: O08736

  • Positive control
    • WB: Human heart lysate. IHC-P: Human and rat heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38-45 kDa (predicted molecular weight: 38-45 kDa). This antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 kDa) in the appropriate lysates.
IHC-P Use a concentration of 2 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 18621034
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

Anti-Caspase-12 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (ab62484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (ab62484)

    Paraffin-embedded rat heart tissue stained for Caspase-12 using ab62484 at 2.5 µg/ml dilution in immunohistochemical analysis.

  • Western blot - Anti-Caspase-12 antibody (ab62484)
    Western blot - Anti-Caspase-12 antibody (ab62484)
    Lane 1 : Anti-Caspase-12 antibody (ab62484) at 0.5 µg/ml
    Lane 2 : Anti-Caspase-12 antibody (ab62484) at 1 µg/ml
    Lane 3 : Anti-Caspase-12 antibody (ab62484) at 2 µg/ml

    Lane 1 : Human heart lysate
    Lane 2 : Human heart lysate
    Lane 3 : Human heart lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Anti rabbit IgG secondary antibody

    Predicted band size : 38-45 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry - Anti-Caspase-12 antibody (ab62484)
    Immunohistochemistry - Anti-Caspase-12 antibody (ab62484)

    Immunohistochemical analysis of human heart tissue labelling Caspase-12 with ab62484 at 10 µg/mL.

  • Western blot - Anti-Caspase-12 antibody (ab62484)
    Western blot - Anti-Caspase-12 antibody (ab62484)This image is courtesy of an abreview submitted by Karine Thibault, CNRS, Paris, France
    All lanes : Anti-Caspase-12 antibody (ab62484) at 1/2000 dilution

    Lane 1 : rat dorsal root ganglion
    Lane 2 : treated rat dorsal root ganglion

    Lysates/proteins at 15 µg per lane.

    Secondary
    anti-rabbit HRP-linked secondary antibody at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38-45 kDa


    Exposure time : 2 minutes

    This image is courtesy of an abreview submitted by Karine Thibault, CNRS, Paris, France

    The full length Casp12 (42 kDa) observed in control rat was cleaved into an active form with a size of 38 kDa in treated rat

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (ab62484)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-12 antibody (ab62484)

    Paraffin-embedded human heart tissue stained for Caspase-12 using ab62484 at 2 µg/ml dilution in immunohistochemical analysis.

References for Anti-Caspase-12 antibody (ab62484)

This product has been referenced in:
  • Zhu G  et al. Endoplasmic reticulum stress mediates distinct impacts of sevoflurane on different subfields of immature hippocampus. J Neurochem 142:272-285 (2017). Read more (PubMed: 28444766) »
  • Wu Z  et al. Silencing of both ATF4 and PERK inhibits cell cycle progression and promotes the apoptosis of differentiating chondrocytes. Int J Mol Med 40:101-111 (2017). IHC ; Mouse . Read more (PubMed: 28498443) »

See all 11 Publications for this product

Publishing research using ab62484? Please let us know so that we can cite the reference in this datasheet.

Product Wall

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11 Abreviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-Caspase-12 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2 cell line)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycine gel)
Loading amount
50000 cells
Specification
HEPG2 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
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Abcam user community

Verified customer

Submitted Apr 25 2017

Western blot abreview for Anti-Caspase-12 antibody

 Below Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (MOLT4)
Gel Running Conditions
Reduced Denaturing (4-20% gradient)
Loading amount
100000 cells
Treatment
Bortezomib 0-20hours
Specification
MOLT4
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Username

Abcam user community

Verified customer

Submitted Jun 19 2015

Western blot abreview for Anti-Caspase-12 antibody

 Good
Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (LIVER)
Specification
LIVER
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
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Username

Abcam user community

Verified customer

Submitted Nov 10 2014

WB with hu, ms, rat
ab62484, ab62463: do they recognize the cleaved caspase 12? at which MW was a band observed?

Thank you for contacting us.

The lab let me know the following:

Both antibodies should recognize the pro form and the fragment downstream of the K158/T159 cleavage site. ab62484 detects a band about 45kDa in human heart and ab62463...

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Only recived 20ul, instead of 100ul.

Thank you for calling Abcam.
I am sorry that the vial of ab62484 that you received was short and I am sending you another vial to make up for that. Your new order number is *.
If there is anything else I can help you with, please let me know.

Please see customers inquiry below:
I was wondering how effectively these will work on human skeletal muscle biopsy samples. I can’t seem to see an article where these are used under those conditions.

ab32539

ab32125

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Thank you for your inquiry.
I am happy to confirm that all these antibodies are tested and guaranteed to work with human samples.
You are correct that we do not have any reports yet about the use of these antibodies in muscle. I assume that i...

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I bought an primary antibody , anti-caspase 12 antibody(ab62484) several months ago from your company . I used them for IHC-P of rats' chondcyte. I have positive results , but now I have a question. Since this antibody reacts with both the propeptide a...

Read More

Thank you for contacting us.

Usinga single antibody, the only way to distinguish between the pro and cleaved forms of Caspase 12 would be by the molecular weight in a western blot. The full length pro Caspase 12 will run at ˜42 kDa, while the...

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Thank you for your reply. I wonder if it's possible that I could use the other caspase-12 peptides available? I just want to do preabsorption for this Anti-caspase 12 primary antibody (ab62848). Thanks again!

Thank you for your reply.

Regrettably, I canconfirm that we donotsell the specific blocking peptide for this antibody.

I am sorry we do not have the product to meet your requirements on this occasion. If you have any further quest...

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Dear Sir/Madam Please kindly let me know. Regarding, Anti-Caspase 12 antibody (ab62484) information about Specificity : This antibody reacts with both the propeptide and cleaved forms of Caspase 12 , It mean can detect the sample is full length and ...

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Thank you for your inquiry. I can confirm that the immunogen sequence is downstream of the cleavage site at approximately residue 120. This means the antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 ...

Read More

Western blot abreview for Anti-Caspase 12 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Dorsal root ganglion)
Loading amount
15 µg
Specification
Dorsal root ganglion
Gel Running Conditions
Reduced Denaturing (gel 10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
abreview image
Username

Dr. Karine Thibault

Verified customer

Submitted Oct 13 2010

1-10 of 11 Abreviews or Q&A

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