Overview

  • Product nameAnti-Caspase-12 antibody
    See all Caspase-12 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-12
  • SpecificityThis antibody reacts with both the propeptide and cleaved forms of Caspase-12.
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-12. immunogen sequence from within aa 181 to 240
    Database link: O08736

  • Positive control
    • Human heart lysate and human heart tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab62484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38-45 kDa (predicted molecular weight: 38-45 kDa). This antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 kDa) in the appropriate lysates.
IHC-P Use a concentration of 2 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

Anti-Caspase-12 antibody images

  • Lane 1 : Anti-Caspase-12 antibody (ab62484) at 0.5 µg/ml
    Lane 2 : Anti-Caspase-12 antibody (ab62484) at 1 µg/ml
    Lane 3 : Anti-Caspase-12 antibody (ab62484) at 2 µg/ml

    Lane 1 : Human heart lysate
    Lane 2 : Human heart lysate
    Lane 3 : Human heart lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    Anti rabbit IgG secondary antibody

    Predicted band size : 38-45 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Caspase-12 antibody (ab62484) at 1/2000 dilution

    Lane 1 : rat dorsal root ganglion
    Lane 2 : treated rat dorsal root ganglion

    Lysates/proteins at 15 µg per lane.

    Secondary
    anti-rabbit HRP-linked secondary antibody at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38-45 kDa


    Exposure time : 2 minutes

    This image is courtesy of an abreview submitted by Karine Thibault, CNRS, Paris, France

    The full length Casp12 (42 kDa) observed in control rat was cleaved into an active form with a size of 38 kDa in treated rat

    See Abreview

  • Immunohistochemical analysis of human heart tissue labelling Caspase-12 with ab62484 at 10 µg/mL.

  • Immunohistochemical staining of human heart tissue using ab62484 antibody at 2 µg/ml.

References for Anti-Caspase-12 antibody (ab62484)

This product has been referenced in:
  • Choudhury S  et al. Caspase-7 ablation modulates UPR, reprograms TRAF2-JNK apoptosis and protects T17M rhodopsin mice from severe retinal degeneration. Cell Death Dis 4:e528 (2013). Mouse . Read more (PubMed: 23470535) »
  • Chis R  et al. a-Crystallin B prevents apoptosis after H2O2 exposure in mouse neonatal cardiomyocytes. Am J Physiol Heart Circ Physiol 303:H967-78 (2012). WB ; Mouse . Read more (PubMed: 22904156) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (MOLT4)
Gel Running Conditions Reduced Denaturing (4-20% gradient)
Loading amount 100000 cells
Treatment Bortezomib 0-20hours
Specification MOLT4
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 19 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (LIVER)
Specification LIVER
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Nov 10 2014

Thank you for contacting us.

The lab let me know the following:

Both antibodies should recognize the pro form and the fragment downstream of the K158/T159 cleavage site. ab62484 detects a band about 45kDa in human heart and ab62463...

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Thank you for calling Abcam.
I am sorry that the vial of ab62484 that you received was short and I am sending you another vial to make up for that. Your new order number is *.
If there is anything else I can help you with, please let me know.

Thank you for your inquiry.
I am happy to confirm that all these antibodies are tested and guaranteed to work with human samples.
You are correct that we do not have any reports yet about the use of these antibodies in muscle. I assume that i...

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Thank you for contacting us.

Usinga single antibody, the only way to distinguish between the pro and cleaved forms of Caspase 12 would be by the molecular weight in a western blot. The full length pro Caspase 12 will run at ˜42 kDa, while the...

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Thank you for your reply.

Regrettably, I canconfirm that we donotsell the specific blocking peptide for this antibody.

I am sorry we do not have the product to meet your requirements on this occasion. If you have any further quest...

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Thank you for your inquiry. I can confirm that the immunogen sequence is downstream of the cleavage site at approximately residue 120. This means the antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Dorsal root ganglion)
Loading amount 15 µg
Specification Dorsal root ganglion
Gel Running Conditions Reduced Denaturing (gel 10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Karine Thibault

Verified customer

Submitted Oct 13 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (MCF-7)
Specification MCF-7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jun 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"