Overview

  • Product name
  • Description
    Rabbit polyclonal to Caspase-12
  • Host species
    Rabbit
  • Specificity
    This antibody reacts with both the propeptide and cleaved forms of Caspase-12.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-12. immunogen sequence from within aa 181 to 240
    Database link: O08736

  • Positive control
    • WB: Human heart lysate. IHC-P: Human and rat heart tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38-45 kDa (predicted molecular weight: 38-45 kDa). This antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 kDa) in the appropriate lysates.
IHC-P Use a concentration of 2 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 18621034
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

Images

  • Paraffin-embedded rat heart tissue stained for Caspase-12 using ab62484 at 2.5 µg/ml dilution in immunohistochemical analysis.

  • Lane 1 : Anti-Caspase-12 antibody (ab62484) at 0.5 µg/ml
    Lane 2 : Anti-Caspase-12 antibody (ab62484) at 1 µg/ml
    Lane 3 : Anti-Caspase-12 antibody (ab62484) at 2 µg/ml

    All lanes : Human heart lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Anti rabbit IgG secondary antibody

    Predicted band size: 38-45 kDa
    Observed band size: 45 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analysis of human heart tissue labelling Caspase-12 with ab62484 at 10 µg/mL.

  • All lanes : Anti-Caspase-12 antibody (ab62484) at 1/2000 dilution

    Lane 1 : rat dorsal root ganglion
    Lane 2 : treated rat dorsal root ganglion

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : anti-rabbit HRP-linked secondary antibody at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 38-45 kDa


    Exposure time: 2 minutes


    The full length Casp12 (42 kDa) observed in control rat was cleaved into an active form with a size of 38 kDa in treated rat

    See Abreview

  • Paraffin-embedded human heart tissue stained for Caspase-12 using ab62484 at 2 µg/ml dilution in immunohistochemical analysis.

References

This product has been referenced in:
  • Meng C  et al. PERK Pathway Activation Promotes Intracerebral Hemorrhage Induced Secondary Brain Injury by Inducing Neuronal Apoptosis Both in Vivo and in Vitro. Front Neurosci 12:111 (2018). Read more (PubMed: 29541018) »
  • Feng Y  et al. Selective Histone Deacetylase 6 Inhibitor 23BB Alleviated Rhabdomyolysis-Induced Acute Kidney Injury by Regulating Endoplasmic Reticulum Stress and Apoptosis. Front Pharmacol 9:274 (2018). Read more (PubMed: 29632491) »

See all 26 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEPG2 cell line)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycine gel)
Loading amount
50000 cells
Specification
HEPG2 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 25 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (MOLT4)
Gel Running Conditions
Reduced Denaturing (4-20% gradient)
Loading amount
100000 cells
Treatment
Bortezomib 0-20hours
Specification
MOLT4
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 19 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (LIVER)
Specification
LIVER
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Nov 10 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Dorsal root ganglion)
Loading amount
15 µg
Specification
Dorsal root ganglion
Gel Running Conditions
Reduced Denaturing (gel 10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Karine Thibault

Verified customer

Submitted Oct 13 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MCF-7)
Specification
MCF-7
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jun 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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