• Product name
  • Description
    Rabbit polyclonal to Caspase-12
  • Specificity
    This antibody reacts with both the propeptide and cleaved forms of Caspase-12.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-12. immunogen sequence from within aa 181 to 240
    Database link: O08736

  • Positive control
    • WB: Human heart lysate. IHC-P: Human and rat heart tissue.



Our Abpromise guarantee covers the use of ab62484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38-45 kDa (predicted molecular weight: 38-45 kDa). This antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 kDa) in the appropriate lysates.
IHC-P Use a concentration of 2 µg/ml.
IHC-Fr Use at an assay dependent concentration. PubMed: 18621034
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.


Anti-Caspase-12 antibody images

  • Paraffin-embedded rat heart tissue stained for Caspase-12 using ab62484 at 2.5 µg/ml dilution in immunohistochemical analysis.

  • Lane 1 : Anti-Caspase-12 antibody (ab62484) at 0.5 µg/ml
    Lane 2 : Anti-Caspase-12 antibody (ab62484) at 1 µg/ml
    Lane 3 : Anti-Caspase-12 antibody (ab62484) at 2 µg/ml

    Lane 1 : Human heart lysate
    Lane 2 : Human heart lysate
    Lane 3 : Human heart lysate

    Lysates/proteins at 15 µg per lane.

    Anti rabbit IgG secondary antibody

    Predicted band size : 38-45 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of human heart tissue labelling Caspase-12 with ab62484 at 10 µg/mL.

  • All lanes : Anti-Caspase-12 antibody (ab62484) at 1/2000 dilution

    Lane 1 : rat dorsal root ganglion
    Lane 2 : treated rat dorsal root ganglion

    Lysates/proteins at 15 µg per lane.

    anti-rabbit HRP-linked secondary antibody at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38-45 kDa

    Exposure time : 2 minutes

    This image is courtesy of an abreview submitted by Karine Thibault, CNRS, Paris, France

    The full length Casp12 (42 kDa) observed in control rat was cleaved into an active form with a size of 38 kDa in treated rat

    See Abreview

  • Paraffin-embedded human heart tissue stained for Caspase-12 using ab62484 at 2 µg/ml dilution in immunohistochemical analysis.

References for Anti-Caspase-12 antibody (ab62484)

This product has been referenced in:
  • Zhu G  et al. Endoplasmic reticulum stress mediates distinct impacts of sevoflurane on different subfields of immature hippocampus. J Neurochem 142:272-285 (2017). Read more (PubMed: 28444766) »
  • Wu Z  et al. Silencing of both ATF4 and PERK inhibits cell cycle progression and promotes the apoptosis of differentiating chondrocytes. Int J Mol Med 40:101-111 (2017). IHC ; Mouse . Read more (PubMed: 28498443) »

See all 11 Publications for this product

Product Wall

Thank you for contacting us.

The lab let me know the following:

Both antibodies should recognize the pro form and the fragment downstream of the K158/T159 cleavage site. ab62484 detects a band about 45kDa in human heart and ab62463...

Read More

Thank you for calling Abcam.
I am sorry that the vial of ab62484 that you received was short and I am sending you another vial to make up for that. Your new order number is *.
If there is anything else I can help you with, please let me know.

Thank you for your inquiry.
I am happy to confirm that all these antibodies are tested and guaranteed to work with human samples.
You are correct that we do not have any reports yet about the use of these antibodies in muscle. I assume that i...

Read More

Thank you for contacting us.

Usinga single antibody, the only way to distinguish between the pro and cleaved forms of Caspase 12 would be by the molecular weight in a western blot. The full length pro Caspase 12 will run at ˜42 kDa, while the...

Read More

Thank you for your reply.

Regrettably, I canconfirm that we donotsell the specific blocking peptide for this antibody.

I am sorry we do not have the product to meet your requirements on this occasion. If you have any further quest...

Read More

Thank you for your inquiry. I can confirm that the immunogen sequence is downstream of the cleavage site at approximately residue 120. This means the antibody will detect full length Casp12 (42 kDa) and also the cleaved active form (38 ...

Read More


Sign up