Overview

  • Product nameAnti-Caspase-12 antibody
    See all Caspase-12 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-12
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Cow, Dog, Human, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-12 aa 400 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q6UXS9
    (Peptide available as ab98317, ab98329, ab98329)

  • Positive control
    • This antibody gave a positive signal in Mouse and Rat Spleen tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab87348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).

Target

Anti-Caspase-12 antibody images

  • All lanes : Anti-Caspase-12 antibody (ab87348) at 1 µg/ml

    Lane 1 : Spleen (Rat) Tissue Lysate
    Lane 2 : Spleen (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

References for Anti-Caspase-12 antibody (ab87348)

ab87348 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Dog Cell lysate - whole cell (MDCK)
Specification MDCK
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 28 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (rat podocyte)
Loading amount 30 µg
Specification rat podocyte
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

Submitted Apr 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"