Overview

  • Product nameCaspase 12 Assay Kit (Fluorometric)
    See all Caspase 12 kits
  • Tests
    100 x 1 assay
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Caspase 12 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (?max = 400 nm); upon cleavage of the substrate by caspase 12 or related caspases, free AFC emits a yellow-green fluorescence (?max = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
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  • Notes

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • RelevanceCaspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. Caspase 12 is most highly related to members of the ICE subfamily. However, human caspase 12 has no protease activity. It may reduce cytokine release in response to bacterial lipopolysaccharide during infections and reduces activation of NF-kappa-B in response to TNF.
  • Alternative names
    • CASP12
    • CASP12P1
    • Caspase 12
    • Inactive caspase 12

Associated products

Applications

Our Abpromise guarantee covers the use of ab65664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Protocols

References for Caspase 12 Assay Kit (Fluorometric) (ab65664)

This product has been referenced in:
  • Mitra A  et al. Cardioprotective role of P38 MAPK during myocardial infarction via parallel activation of a-crystallin B and Nrf2. J Cell Physiol 229:1272-82 (2014). Functional Studies . Read more (PubMed: 24464634) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"