Caspase 2 (active) FITC Staining Kit (ab65612)

Overview

  • Product nameCaspase 2 (active) FITC Staining Kit
    See all Caspase 2 kits
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Caspase 2 (active) FITC Staining Kit provides a convenient means for sensitive detection of activated caspase 2 in living cells. The assay utilizes the caspase 2 inhibitor, VDVAD-FMK, conjugated to FITC (FITC-VDVAD-FMK) as a marker. FITC-VDVAD-FMK is cell permeable, non-toxic, and irreversibly binds to activated caspase 2 in apoptotic cells. The FITC label allows detection of activated caspase 2 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of caspases plays a central role in apoptosis.

  • Tested applicationsSuitable for: FM, Functional Studies, Flow Cytmore details

Properties

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
  • Tissue specificityExpressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The mature protease can process its own propeptide, but not that of other caspases.
  • Information by UniProt
  • Alternative names
    • CASP-2
    • Casp2
    • CASP2_HUMAN
    • Caspase-2 subunit p12
    • Caspase2
    • ICH 1L
    • ICH1
    • ICH1 protease
    • ICH1L
    • ICH1L/1S
    • NEDD-2
    • NEDD2
    • Neural precursor cell expressed developmentally down-regulated protein 2
    • Protease ICH-1
    see all

Associated products

Applications

Our Abpromise guarantee covers the use of ab65612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Functional Studies Use at an assay dependent dilution.
Flow Cyt Use at an assay dependent dilution.

Protocols

References for Caspase 2 (active) FITC Staining Kit (ab65612)

ab65612 has not yet been referenced specifically in any publications.

Product Wall

The developer of the kit suggests trypsinizing the cells and then using the protocol for cells in suspension. If you do the assay this way, it would probably be best any apply the apoptosis-inducing treatment to the cells after trypsinization, to the c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"