Anti-Caspase-2 antibody (ab182657)
Key features and details
- Rabbit polyclonal to Caspase-2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Caspase-2 antibody -
Description
Rabbit polyclonal to Caspase-2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human Caspase-2 aa 1-250. BC002427
Database link: P42575 -
Positive control
- Human fetal kidney and lung tissue lysate; Human urinary bladder tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/300.
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WB |
1/1000 - 1/2000. Predicted molecular weight: 51 kDa.
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Notes |
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IHC-P
1/100 - 1/300. |
WB
1/1000 - 1/2000. Predicted molecular weight: 51 kDa. |
Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. -
Tissue specificity
Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe mature protease can process its own propeptide, but not that of other caspases. - Information by UniProt
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Database links
- Entrez Gene: 835 Human
- Entrez Gene: 12366 Mouse
- Entrez Gene: 64314 Rat
- Omim: 600639 Human
- SwissProt: P42575 Human
- SwissProt: P29594 Mouse
- SwissProt: P55215 Rat
- Unigene: 368982 Human
see all -
Alternative names
- Casp 2L Pro antibody
- CASP-2 antibody
- Casp2 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human urinary bladder tissue labeling Caspase 2 using ab182657 at 1/100 dilution. The right panel is treated with the fusion protein.
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All lanes : Anti-Caspase-2 antibody (ab182657) at 1/950 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal lung lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution
Predicted band size: 51 kDa
Exposure time: 2 minutes10% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab182657 has not yet been referenced specifically in any publications.