Validated using a knockout cell line
Recombinant
RabMAb

Anti-Caspase-2 antibody [EPR16796] (ab179520)

Overview

  • Product name
    Anti-Caspase-2 antibody [EPR16796]
    See all Caspase-2 primary antibodies
  • Description
    Rabbit monoclonal [EPR16796] to Caspase-2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse Caspase-2 aa 150-350. The exact sequence is proprietary.
    Database link: P29594

  • Positive control
    • WB: HeLa whole cell lysates treated with 4uM camptothecin for 4 hours; C6 whole cell lysates treated with 1uM staurosporine for 4 hours; RAW 264.7 whole cell lysate; Human fetal kidney lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab179520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 48, 33 kDa (predicted molecular weight: 51 kDa).

Target

  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
  • Tissue specificity
    Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The mature protease can process its own propeptide, but not that of other caspases.
  • Information by UniProt
  • Database links
  • Alternative names
    • CASP 2 antibody
    • CASP-2 antibody
    • Casp2 antibody
    • CASP2_HUMAN antibody
    • Caspase 2 antibody
    • Caspase 2 apoptosis related cysteine peptidase antibody
    • Caspase-2 subunit p12 antibody
    • Caspase2 antibody
    • ICH 1 antibody
    • ICH 1 protease antibody
    • ICH 1L antibody
    • ICH1 antibody
    • ICH1 protease antibody
    • ICH1L antibody
    • NEDD-2 antibody
    • NEDD2 antibody
    • Neural precursor cell expressed developmentally down-regulated protein 2 antibody
    • PPP1R57 antibody
    • Protease ICH-1 antibody
    • Protein phosphatase 1 regulatory subunit 57 antibody
    see all

Images



  • Predicted band size : 51 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Caspase-2 knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab17952 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.

    ab179520 was shown to recognize Caspase-2 when Caspase-2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Caspase-2 knockout samples were subjected to SDS-PAGE. ab179520 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 4uM camptothecin for 4 hours
    Lane 2 : Untreated HeLa whole cell lysate
    Lane 3 : C6 (Rat glial tumor cells) whole cell lysate treated with 1uM staurosporine for 4 hours
    Lane 4 : Untreated C6 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 51 kDa
    Observed band size : 33,48 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    The 33KDa band (cleaved caspase 2) contains subunit p18.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/1000 dilution + RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 51 kDa
    Observed band size : 33,48 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    The 33KDa band (cleaved caspase 2) contains subunit p18.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Caspase-2 antibody [EPR16796] (ab179520) at 1/2000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 51 kDa


    Exposure time : 3 minutes

    The 33KDa band (cleaved caspase 2) contains subunit p18.

    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:
  • Liu J  et al. BECN1-dependent CASP2 incomplete autophagy induction by binding to rabies virus phosphoprotein. Autophagy 13:739-753 (2017). Read more (PubMed: 28129024) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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