Overview

  • Product nameAnti-Caspase-2 antibody [Y154]
    See all Caspase-2 primary antibodies
  • Description
    Rabbit monoclonal [Y154] to Caspase-2
  • SpecificityThis antibody is specific for the pro form and the p18 cleaved form of Caspase-2.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-2 aa 250-350 (C terminal).
    Database link: P42575

  • Positive control
    • Jurkat cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).

Detects bands of approximately 50 kDa (pro form) and 18 kDa (p18 cleaved form).

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
  • Tissue specificityExpressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The mature protease can process its own propeptide, but not that of other caspases.
  • Information by UniProt
  • Database links
  • Alternative names
    • CASP 2 antibody
    • CASP-2 antibody
    • Casp2 antibody
    • CASP2_HUMAN antibody
    • Caspase 2 antibody
    • Caspase 2 apoptosis related cysteine peptidase antibody
    • Caspase-2 subunit p12 antibody
    • Caspase2 antibody
    • ICH 1 antibody
    • ICH 1 protease antibody
    • ICH 1L antibody
    • ICH1 antibody
    • ICH1 protease antibody
    • ICH1L antibody
    • NEDD-2 antibody
    • NEDD2 antibody
    • Neural precursor cell expressed developmentally down-regulated protein 2 antibody
    • PPP1R57 antibody
    • Protease ICH-1 antibody
    • Protein phosphatase 1 regulatory subunit 57 antibody
    see all

Anti-Caspase-2 antibody [Y154] images



  • Predicted band size : 49 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Caspase 2 knockout HAP1 cell lysate (20 µg)

    Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.
    ab32021 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab32021 and ab8226 (loading control to beta actin) were diluted 1/50 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Anti-Caspase-2 antibody [Y154] (ab32021) at 1/500 dilution + Jurkat cell lysate.

    Predicted band size : 49 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa (possible cleavage fragment).

References for Anti-Caspase-2 antibody [Y154] (ab32021)

This product has been referenced in:
  • Shen Q  et al. Regulation of CRADD-caspase 2 cascade by histone deacetylase 1 in gastric cancer. Am J Transl Res 6:538-47 (2014). Read more (PubMed: 25360218) »
  • Yoon JY  et al. Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B. Blood Cancer J 3:e153 (2013). Human, Human, Human, Human . Read more (PubMed: 24141622) »

See all 6 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"