The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
Detects bands of approximately 50 kDa (pro form) and 18 kDa (p18 cleaved form).
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
Tissue specificityExpressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
Sequence similaritiesBelongs to the peptidase C14A family. Contains 1 CARD domain.
Post-translational modificationsThe mature protease can process its own propeptide, but not that of other caspases.
Caspase 2 apoptosis related cysteine peptidase antibody
Caspase-2 subunit p12 antibody
ICH 1 antibody
ICH 1 protease antibody
ICH 1L antibody
ICH1 protease antibody
Neural precursor cell expressed developmentally down-regulated protein 2 antibody
Protease ICH-1 antibody
Protein phosphatase 1 regulatory subunit 57 antibody
Anti-Caspase-2 antibody [Y154] images
Western blot - Anti-Caspase-2 antibody [Y154] (ab32021)
Predicted band size : 49 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: Caspase 2 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa. ab32021 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab32021 and ab8226 (loading control to beta actin) were diluted 1/50 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-Caspase-2 [Y154] antibody (ab32021)
Yoon JY et al. Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B. Blood Cancer J3:e153 (2013).
Human, Human, Human, Human
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