Overview

  • Product nameCaspase 2 Assay Kit (Colorimetric)
    See all Caspase 2 kits
  • Tests
    100 x 1 assay
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Caspase 2 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate VDVAD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • Tested applicationsFunctional Studiesmore details

Properties

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.
  • Tissue specificityExpressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The mature protease can process its own propeptide, but not that of other caspases.
  • Information by UniProt
  • Alternative names
    • Apoptosis related cysteine peptidase
    • CASP 2
    • Casp 2L Pro
    • CASP-2
    • Casp2
    • CASP2_HUMAN
    • Caspase 2 apoptosis related cysteine protease
    • Caspase-2 subunit p12
    • Caspase2
    • Developmentally down regulated 2
    • ICH 1
    • ICH 1 protease
    • ICH 1L
    • ICH 1L/1S
    • ICH1
    • ICH1 protease
    • ICH1L
    • ICH1L/1S
    • NEDD 2
    • NEDD-2
    • NEDD2
    • NEDD2 apoptosis regulatory gene
    • Neural precursor cell expressed
    • Neural precursor cell expressed developmentally down regulated 2
    • Neural precursor cell expressed developmentally down-regulated protein 2
    • Protease ICH-1
    see all

Applications

Our Abpromise guarantee covers the use of ab39830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Protocols

References for Caspase 2 Assay Kit (Colorimetric) (ab39830)

ab39830 has not yet been referenced specifically in any publications.

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