Overview

  • Product nameCaspase 2 Assay Kit (Colorimetric)See all Caspase 2 kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Tissue Extracts, Cell Lysate
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Caspase 2 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate VDVAD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
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  • Notes

    Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCaspase 2 has a positive and a negative role in the regulation of the apoptotic process. Overexpression of the full length Caspase 2 (Nedd2/Ich1 ) mRNA in some cell types results in apoptosis, whereas overexpression of an alternative Caspase 2 splice variant suppresses apoptosis induced by serum withdrawal. Caspase 2 may be activated in vitro by Caspase 1, Caspase 3 and the neutral serine protease granzyme B which is stored in the specialized lytic granules of cytotoxic lymphocytes.
  • Cellular localizationCytoplasmic
  • Alternative names
    • Apoptosis related cysteine peptidase
    • CASP 2
    • Casp 2L Pro
    • CASP2
    • Caspase 2 apoptosis related cysteine protease
    • Caspase2
    • Developmentally down regulated 2
    • ICH 1
    • ICH 1 protease
    • ICH 1L
    • ICH 1L/1S
    • ICH1
    • ICH1 protease
    • ICH1L
    • ICH1L/1S
    • NEDD 2
    • NEDD2
    • NEDD2 apoptosis regulatory gene
    • Neural precursor cell expressed
    • Neural precursor cell expressed developmentally down regulated 2
    see all

Applications

Our Abpromise guarantee covers the use of ab39830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent concentration.

Protocols

References for Caspase 2 Assay Kit (Colorimetric) (ab39830)

ab39830 has not yet been referenced specifically in any publications.

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