Overview

  • Product nameAnti-Caspase-2L antibody
    See all Caspase-2L primary antibodies
  • Description
    Rabbit polyclonal to Caspase-2L
  • Specificityab18737 detects the full length and small subunit of caspase-2. In human samples it also detects a 30kDa intermediate fragment.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse Caspase-2L. Immuogen: Synthetic peptide surrounding amino acid 417 of mouse caspase-2
    Database link: P42575
    (Peptide available as ab38266)

  • Positive control
    • Mouse small intestine lysate. This antibody gave a positive result in IF/ICC when used in the following formaldehyde fixed cell lines: MCF-7.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab18737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).Can be blocked with Mouse Caspase-2L peptide (ab38266). Full length protein and a smaller band of about 12 kDa (small subunit).

Target

  • RelevanceFunction: Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Tissue specificity: Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. PTM: The mature protease can process its own propeptide, but not that of other caspases.
  • Database links
  • FormSpecific for P42575-1; the isoform that has been chosen as the 'canonical' sequence.
  • Alternative names
    • CASP2 antibody
    • ICH-1L antibody
    • ICH1 antibody
    • NEDD-2 antibody
    • NEDD2 antibody
    • Protease ICH-1 antibody
    see all

Anti-Caspase-2L antibody images



  • Predicted band size : 49 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 12 kDa (possible cleavage fragment).

    WB of Caspase-2 detected with ab18737. Lanes 1 and 2: untreated cell lysate Lanes 3 and 4: treated cell lysate

  • ICC/IF image of ab18737 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab18737 at 5µg/ml overnight at +4°C. The secondary antibody (green) was anti-rabbit DyLight® 488 (ab96899) used at a 1:1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Caspase-2L antibody (ab18737)

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12% gel, MOPS buffer)
Sample Mouse Cell lysate - whole cell (C17.2 cell line)
Specification C17.2 cell line
Treatment Heat-shock for 2 hours at 43 degrees
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2013

Thank you for contacting us.

1. The alternative to Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001 is ab96871; http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-ab96871.html

or ab96879; http:...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"