Recombinant
RabMAb

Anti-Caspase-2L antibody [EPR16790] (ab179519)

Overview

  • Product name
    Anti-Caspase-2L antibody [EPR16790]
    See all Caspase-2L primary antibodies
  • Description
    Rabbit monoclonal [EPR16790] to Caspase-2L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Mouse Caspase-2L aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P29594

  • Positive control
    • WB: Jurkat, NIH/3T3 and C6 whole cell lysates. IHC-P: Human testis and lung cancer tissue. ICC/IF: HeLa cells. Flow cytometry: Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 12, 14, 30, 48 kDa (predicted molecular weight: 51 kDa).
ICC/IF 1/500.
Flow Cyt 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. Tissue specificity: Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. PTM: The mature protease can process its own propeptide, but not that of other caspases.
  • Database links
  • Form
    Specific for P42575-1; the isoform that has been chosen as the 'canonical' sequence.
  • Alternative names
    • CASP2 antibody
    • ICH-1L antibody
    • ICH1 antibody
    • NEDD-2 antibody
    • NEDD2 antibody
    • Protease ICH-1 antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Caspase-2L with ab179519 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HeLa cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab179519 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

     

  • All lanes : Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/2000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates treated with 1uM staurosporine for 4 hours
    Lane 2 : untreated Jurkat whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Observed MW: 48, 30, 14, 12 kDa

  • Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/2000 dilution + NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Observed MW: 48,30,12 kDa

  • Anti-Caspase-2L antibody [EPR16790] (ab179519) at 1/10000 dilution + C6 (Rat glial tumor cells) whole cell lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Observed MW: 48,12 kDa

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Caspase-2L using ab179519 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab179519.
    Note: nuclear staining on the germ cells of the Human testis was observed.

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Caspase-2L using ab179519 at 1/250 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab179519.
    Note: nuclear staining on the tumor cells of Human lung cancer was observed.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Caspase-2 with ab179519 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

ab179519 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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