• Product name
    Anti-Caspase-3 antibody [31A893]
    See all Caspase-3 primary antibodies
  • Description
    Mouse monoclonal [31A893] to Caspase-3
  • Specificity
    ab13586 clone 31A893 detects pro-Caspase-3 and active cleaved Caspase-3.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Caspase-3.
    Database link: P42574

  • Positive control
    • HL60 lysates, human breast tissue, Jurkat cells.



Our Abpromise guarantee covers the use of ab13586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml.
IHC-P Use at an assay dependent concentration.


  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • Tissue specificity
    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similarities
    Belongs to the peptidase C14A family.
  • Post-translational
    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all


  • All lanes : Anti-Caspase-3 antibody [31A893] (ab13586)

    Lane 1 : Jurkat cells treated with 2 µM staurosporine
    Lane 2 : Jurkat cells treated with 2 µM staurosporine
    Lane 3 : Jurkat cells treated with 2 µM staurosporine
    Lane 4 : Jurkat cells treated with 2 µM staurosporine
    Lane 5 : Jurkat cells treated with 2 µM staurosporine
    Lane 6 : Jurkat cells treated with 2 µM staurosporine
  • Immunohistochemical analysis of formalin-fixed, paraffin embedded human breast tissue labeling Caspase-3 with ab13586 at 5 µg/ml. Very strong diffused as well as granular immunopositivity of Caspase-3 was observed specifically in the cytoplasmic of ductal /acinar epithelial cells.

  • Anti-Caspase-3 antibody [31A893] (ab13586) at 2 µg/ml + HL60 lysates


This product has been referenced in:
  • Maurya SK  et al. Expression of procaspase 3 and activated caspase 3 and its relevance in hormone-responsive gallbladder carcinoma chemotherapy. Korean J Intern Med 28:573-8 (2013). WB ; Human . Read more (PubMed: 24009453) »
  • Catuogno S  et al. miR-34c may protect lung cancer cells from paclitaxel-induced apoptosis. Oncogene : (2012). WB ; Human . Read more (PubMed: 22370637) »

See all 2 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

The antibody would detect both pro as well as cleaved caspase 3. This product has yet to be tested in ICC/IF. Please feel free to contact us for 100% Abtrial discount if you are interested in testing this product. ...

Read More


Sign up