Overview

  • Product nameAnti-Caspase-3 antibody [3CSP03]
    See all Caspase-3 primary antibodies
  • Description
    Mouse monoclonal [3CSP03] to Caspase-3
  • SpecificityThis antibody is specific to a 32 kDa protein, which is identified as Caspase-3.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Rabbit, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Caspase-3.
    Database link: P42574

  • Positive control
    • Tonsil

Properties

Applications

Our Abpromise guarantee covers the use of ab2171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/75.
IHC-Fr 1/25 - 1/75. ABC method.
IP 1/100 - 1/500.
WB 1/100 - 1/500.

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • Tissue specificityHighly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similaritiesBelongs to the peptidase C14A family.
  • Post-translational
    modifications
    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC 3.4.22.56 antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Anti-Caspase-3 antibody [3CSP03] images

  • ab2171 staining human tonsil by IHC-P.
  • ab2171 staining Caspase-3 in formalin-fixed, paraffin-embedded human tonsil tissue by Immunohistochemistry.

  • Immunohistochemistry (Frozen sections) analysis of rabbit cornea tissue (on day 4, 10 and 15 after the injury) labelling Caspase-3 with ab2171. E − corneal epithelium. a – Caspase-3 staining is strong in the epithelium (particularly in superficial layers, arrows) of injured corneas on day 4 (a) as well as 10 (b) after the injury (arrows). c – On day 15 after the injury the expression of caspase-3 is present in the corneal epithelium as well as in the corneal stroma (arrows show to the cell apoptosis). The sections are counterstained with haematoxylin. Scale bar: 10 μM.

  • Immunohistochemistry (Frozen sections) analysis of rabbit cornea tissue (on day 4, 10 and 15 after the injury treated with MSCs) labelling Caspase-3 with ab2171. E − corneal epithelium. d – The expression of caspase-3 is reduced in the epithelium (arrows) of injured corneas treated with MSCs on nanofiber scaffolds from day 4 (d), day 10 (e) as well as day 15 (f) after the injury. Solely apoptosis (arrows) are seen. The sections are counterstained with haematoxylin.

  • Immunohistochemistry (Frozen sections) analysis of rabbit cornea tissue labelling Caspase-3 with ab2171. g - Caspase-3 expression is absent in a control cornea. h – The primary antibody is omitted from the incubation medium. The section is stained by counterstaining only (as a negative control for caspase-3). E − corneal epithelium.

References for Anti-Caspase-3 antibody [3CSP03] (ab2171)

This product has been referenced in:
  • Li H  et al. Protective effect of ginsenoside Rg1 on lidocaine-induced apoptosis. Mol Med Rep 9:395-400 (2014). WB ; Human . Read more (PubMed: 24270314) »
  • Cejkova J  et al. Suppression of alkali-induced oxidative injury in the cornea by mesenchymal stem cells growing on nanofiber scaffolds and transferred onto the damaged corneal surface. Exp Eye Res 116:312-23 (2013). IHC-Fr ; Rabbit . Read more (PubMed: 24145108) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (Immortalized glioma cell lines)
Gel Running Conditions Reduced Denaturing
Loading amount 50 µg
Specification Immortalized glioma cell lines
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Lisa Schlein

Verified customer

Submitted Sep 09 2015

Thank you for your enquiry regarding ab2171. I can confirm that the average immunoglobulin concentration of Caspase3 (ab2171) is 66 ug/ml. I hope this helps and if I can assist further, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"