Overview

  • Product nameAnti-Caspase-3 antibody [4H334]
    See all Caspase-3 primary antibodies
  • Description
    Rabbit monoclonal [4H334] to Caspase-3
  • SpecificityRecognizes endogenous levels of full length (35kD) and large fragment (17/19kD) of human Caspase-3 resulting from cleavage at (Asp175).
  • Tested applicationsSuitable for: WB, ICC, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human, African Green Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-3 (N terminal). Corresponding to amino-terminal residues adjacent to (Asp175) in human Caspase-3.
    Database link: P42574

Properties

Applications

Our Abpromise guarantee covers the use of ab17819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 33 kDa.
ICC 1/100.
IP 1/100.

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • Tissue specificityHighly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similaritiesBelongs to the peptidase C14A family.
  • Post-translational
    modifications
    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC 3.4.22.56 antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

References for Anti-Caspase-3 antibody [4H334] (ab17819)

This product has been referenced in:
  • Végran F  et al. A short caspase-3 isoform inhibits chemotherapy-induced apoptosis by blocking apoptosome assembly. PLoS One 6:e29058 (2011). WB . Read more (PubMed: 22216167) »
  • Stewart ME & Roy P Role of cellular caspases, nuclear factor-kappa B and interferon regulatory factors in Bluetongue virus infection and cell fate. Virol J 7:362 (2010). WB ; Human . Read more (PubMed: 21134281) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (RAMOS cells)
Specification RAMOS cells
Treatment with or without TGFbeta (50pM, 24h)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

Submitted May 07 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (NMuMG cells)
Specification NMuMG cells
Treatment with or without TGFbeta (50pM, 24h)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

Submitted May 07 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample African Green Monkey Cell lysate - whole cell (COS-1 cells)
Specification COS-1 cells
Treatment with or without TGFbeta (50pM, 24h)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted May 07 2014

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from our Anti-Caspase 3 antibody [4H334] (ab17819) and active Caspase 3 protein (Active) (ab52101) . <...

Read More

Thank you for contacting us again.

I apologize for forgetting your request to a suitable secondary antibody!

The product I would like to suggest is the following:

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L...

Read More

Thank you for contacting us.

I would suggest to use ab17819 Anti-Caspase 3 antibody [4H334]

http://www.abcam.com/index.html?datasheet=17819 (or use the following: http://www.abcam.com/index.html?datasheet=17819).

Even if ...

Read More

According to our records you were experiencing some difficulties with ab17819 and had contacted the Abcam technical team regarding this. Looking at your correspondence we can see that we are awaiting more details from you in order to help you with y...

Read More

Thank you for your enquiry. I am sorry that my response has taken so long. I have been in correspondence with the source of this antibody. It has transpired that this is in fact a Rabbit monoclonal. I have updated our datasheets with immediate affe...

Read More

Thank you for your enquiry. Yes, I think that your choice is the best one. Caspase 3 antibody (ab17819) detects endogenous levels of full length (35kDa) and large fragment (17/19kD) of caspase 3 resulting from cleavage at Asp175. It was derived usi...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"