• Product nameAnti-Caspase-3 antibody
    See all Caspase-3 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-3
  • SpecificityCaspase-3 is a member of the interleukin-1 ß-converting enzyme family. Caspase-3 is thought to be associated with induction of apoptosis. Caspase-3 is synthesized as inactive 32 kDa proenzyme and is processed during apoptosis generating t-o subunits of 17 kDa and 12 kDa. Caspase-3 stains the epithelial cells of skin, renal proximal tubules and collecting ducts.
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-3 aa 167-175. Corresponding to the cleavage site of human caspase 3.
    Database link: P42574

  • Positive control
    • Tonsil



Our Abpromise guarantee covers the use of ab4051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 32 kDa. PubMed: 24367539
IHC-P Use a concentration of 0.002 mg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

IHC-Fr Use at an assay dependent concentration. PubMed: 24465746


  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • Tissue specificityHighly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similaritiesBelongs to the peptidase C14A family.
  • Post-translational
    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Anti-Caspase-3 antibody images

  • Anti-Caspase-3 antibody (ab4051) + Human Lung Cell Extract

    Predicted band size : 32 kDa
  • ab4051 staining Human normal tonsil. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained at room temperature. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked for 10 minutes (block containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin (ab128990)  and coverslipped under DePeX.

  • ab4051 staining Caspase 3 in Pig liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in milk) for 20 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab4051 staining Caspase 3 in Cow Ovary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 15 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/75 in PBS + BSA) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

    See Abreview

  • ab4051 staining Caspase-3 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.

References for Anti-Caspase-3 antibody (ab4051)

This product has been referenced in:
  • Guo J  et al. Berberine Protects Human Umbilical Vein Endothelial Cells against LPS-Induced Apoptosis by Blocking JNK-Mediated Signaling. Evid Based Complement Alternat Med 2016:6983956 (2016). WB ; Human . Read more (PubMed: 27478481) »
  • Qian J  et al. Downregulation of HIF-1a inhibits the proliferation and invasion of non-small cell lung cancer NCI-H157 cells. Oncol Lett 11:1738-1744 (2016). Read more (PubMed: 26998070) »

See all 24 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step None
Sample Cow Tissue sections (Ovary)
Specification Ovary
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Oct 24 2014

1. can it be shipped at room ? ab137994 is shipped on wet ice at 4 degrees C.

2. How long will it be valid after its opening? I am asking because i want to test 20 samples then after 8months test rest of samples..can ...

Read More

Thank you for your enquiry and your interest in our products.

As the datasheet indicates ab4051 has been tested for both IHC-P (paraffin embedded formallin fixed tissues) and IHC-Fr (frozen tissues).

If you need any further assistan...

Read More

Thank you for contacting Abcam.

The concentration of ab4051 lot XXXXXX is XXX mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit mono...

Read More

Thank you for contacting us. I have been in contact with teh lab regarding concnetrations of the antibodies which we spoke of earlier today. AB4051, rabbit polyclonal to Caspase 3, lot xxx has a concentration of xxxxmg/mL AB32152, rabbit monoc...

Read More

ab4051 lot GR78964-1 has a concentration of: 0.31 mg/ml.

Thank you for contacting Abcam regarding ab4051.

The concentration of lot GR54275-1 is 0.3 mg/ml.

I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

Gracias por contactarnos.

Perdona por el retraso en contestar. La concentración del anticuerpo corresponde a 0.31mg/ml.

Espero haberte ayudado. Para cualquier otra consulta, por favor, no dudes en contactarnos de nuevo.

Thank you for contacting Abcam regarding ab833. I have inquired into the origin of this product. This product is not MIB-1. It is a polyclonal which should react against a number of epitope sites on the target protein. This product is covered by ...

Read More

Thank you for your enquiry. I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications and species listed on the datasheets. Therefore, any Caspase 3 antibody tes...

Read More

1-10 of 19 Abreviews or Q&A