Recombinant full length protein corresponding to Human Caspase-3.
Database link: P42574
Our Abpromise guarantee covers the use of ab44976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 32 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20664058|
|IHC-Fr||Use at an assay dependent concentration. See Abreviews|
ab44976, at 1/100, staining Caspase-3 in mouse brain tissue sections by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 5% BSA for 1 hour at 25°C and then incubated with ab44976, for 12 hours at 4°C. A donkey polyclonal to rabbit Ig, diluted 1/500 was used as the secondary antibody. The green shows staining of caspase 3 and the blue DAPI.