Overview

  • Product nameAnti-Caspase-3 antibody
    See all Caspase-3 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-3
  • Specificityab52181 detects endogenous levels of fragment of activated Caspase-3 resulting from cleavage adjacent to Asp175.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-3 aa 126-175.
    Database link: P42574

  • Positive control
    • Human lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab52181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19443712
IHC-P 1/50 - 1/100.
ELISA 1/20000.

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • Tissue specificityHighly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similaritiesBelongs to the peptidase C14A family.
  • Post-translational
    modifications
    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC 3.4.22.56 antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Anti-Caspase-3 antibody images

  • ab52181 at 1/50 dilution staining Caspase-3 in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

    ab52181 at 1/50 dilution staining Caspase 3 in human lung carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References for Anti-Caspase-3 antibody (ab52181)

This product has been referenced in:
  • Ding X  et al. Enhanced HtrA2/Omi expression in oxidative injury to retinal pigment epithelial cells and murine models of neurodegeneration. Invest Ophthalmol Vis Sci 50:4957-66 (2009). ICC/IF . Read more (PubMed: 19443712) »
  • Ding X  et al. Enhanced HtrA2/Omi expression in oxidative injury to retinal pigment epithelial cells and murine models of neurodegeneration. Invest Ophthalmol Vis Sci 50:4957-66 (2009). ICC/IF . Read more (PubMed: 19443712) »

See all 2 Publications for this product

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If the sample of isolated RNA contains activated caspase 3 of human, mouse or rat origin, the antibody would detect it usingone of the tested applications: ICC/IF, IHC-P, ELISA - giv...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (IEC-6)
Specification IEC-6
Fixative Formaldehyde
Permeabilization Yes - 0.1% TX
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
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Verified customer

Submitted Feb 13 2012

Merci de nous avoir contactés. Le laboratoire m'a informé que : L'anti-Caspase 7, référence ab52297, reconnaît les 2 formes, intacte et clivée. Nous aurions possibilité, si vous le souhaitez, d'ajouter &a...

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