• Product name
  • Description
    Rabbit polyclonal to Caspase-3
  • Specificity
    ab52293 detects endogenous levels of fragment of activated Caspase-3 resulting from cleavage adjacent to Asp175.
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-3 aa 140-180 (internal sequence).
    Database link: P42574

  • Positive control
    • IHC: Human lung carcinoma tissue, ICC/IF: HeLa cell line
  • General notes

    This is a Caspase 3 (cleaved-Asp175) antibody.



Our Abpromise guarantee covers the use of ab52293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 17, 32 kDa).
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/20000.


  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • Tissue specificity
    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similarities
    Belongs to the peptidase C14A family.
  • Post-translational
    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Anti-Caspase-3 antibody images

  • Lane 1 : Anti-Caspase-3 antibody (ab52293) (Extracts from 293 cells treated with Etoposide (25 microM, 60 mins))
    Lane 2 : Anti-Caspase-3 antibody (ab52293) (Extracts from 293 cells treated with Etoposide (25 microM, 60 mins) and the CI-peptide)

    Lane 1 : As above
    Lane 2 : As above

    Predicted band size : 17, 32 kDa
  • ICC/IF image of ab52293 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52293, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, anti-rabbit DyLight® 488 used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC- paraffin-embedded human lung carcinoma tissue using ab52293. The image on the right is treated with the synthesized peptide.

References for Anti-Caspase-3 antibody (ab52293)

This product has been referenced in:
  • He X  et al. Atorvastatin protects against contrast-induced nephropathy via anti-apoptosis by the upregulation of Hsp27 in vivo and in vitro. Mol Med Rep 15:1963-1972 (2017). WB . Read more (PubMed: 28260077) »
  • Yao L  et al. Vitamin D attenuates hyperoxia-induced lung injury through downregulation of Toll-like receptor 4. Int J Mol Med 39:1403-1408 (2017). WB ; Rat . Read more (PubMed: 28440468) »

See all 6 Publications for this product

Product Wall

Immunohistochemistry (Frozen sections)
Human Tissue sections (Human skeletal muscle)
Human skeletal muscle
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 02 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate-buffer pH 6,0
Mouse Tissue sections (Tumor)

Abcam user community

Verified customer

Submitted Jul 11 2014

The labels on the vial will sometimes be truncated due to space and printing limitations. The product which you have received is the same as described on the datasheet for ab52293. The expanded product name references the ability of this antibody to re...

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Merci de nous avoir contactés.

Le kit ELISA ab100646 est en effet approprié pour le dosage du TGF-alpha humain contenu dans les supernageants de cultures cellulaires, urine, sérum et plasma.

Pour les anti-Histone ...

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Thank you for your email and letting us know that the IHC-P image was not showing correctly.

This is now fixed. Please review the datasheet.

http://www.abcam.com/index.html?datasheet=52293 (or use the following: http://www.abcam.com...

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Merci de nous avoir contactés. Le laboratoire m'a informé que : L'anti-Caspase 7, référence ab52297, reconnaît les 2 formes, intacte et clivée. Nous aurions possibilité, si vous le souhaitez, d'ajouter &a...

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