Overview

  • Product nameAnti-Caspase-3 antibody
    See all Caspase-3 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Saccharomyces cerevisiae, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human Caspase-3 aa 1-277.
    Sequence:

    MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIII NNKNFHKSTG MTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVE LMRDVSKEDHSKRSSFVCVLLS HGEEGIIFGTNGPVDLKKITNFFRGD RCRSLTGKPKLFIIQACRGTELDCGIETDSGVDD DMACHKIPVEADFL YAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVN RK VATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH


    Database link: P42574

  • Positive control
    • Jurkat cell lysate, Jurkat cells treated with staurosporine (lysate), HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 32 kDa.

A lower molecular weight band refers to a proteolytic cleavage product of caspase-3.

IHC-P Use at an assay dependent concentration.

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • Tissue specificityHighly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similaritiesBelongs to the peptidase C14A family.
  • Post-translational
    modifications
    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC 3.4.22.56 antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Anti-Caspase-3 antibody images

  • All lanes : Anti-Caspase-3 antibody (ab90437) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cells treated with staurosporine
    Lane 3 : MCF-7 cell lysate (negative control)

    Developed using the ECL technique

    Predicted band size : 32 kDa
    Additional bands at : ~18 kDa (possible cleavage fragment),~20 kDa (possible cleavage fragment).

    Western blot analysis of Caspase-3 pAb.

  • Immunohistochemistry analysis of human spleen tissue stained with Caspase-3, pAb at 10µg/ml.

References for Anti-Caspase-3 antibody (ab90437)

This product has been referenced in:
  • Shi W  et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater 45:247-261 (2016). IF ; Human . Read more (PubMed: 27592818) »
  • D'Souza Y  et al. Characterization of Aldh2 (-/-) mice as an age-related model of cognitive impairment and Alzheimer's disease. Mol Brain 8:27 (2015). WB, Dot blot ; Mouse . Read more (PubMed: 25910195) »

See all 8 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Chicken Tissue sections (Neural tube)
Permeabilization No
Specification Neural tube
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Dr. Ayelet Kohl

Verified customer

Submitted Sep 21 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 29 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 29 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Chicken Cell (Embryo)
Permeabilization Yes - 0.1%Tween
Specification Embryo
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. Maneeshi Prasad

Verified customer

Submitted Oct 08 2015

Application Western blot
Sample Mouse Cell lysate - whole cell (mouse hepatocytes)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Treatment 20 ug/m poly(I:C)
Specification mouse hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Sep 01 2015



Here are answers to your questions:

1. Since the immunogen is full length and the antibody is polyclonal this antibody will react with all forms of casepase 3.

2. The 2 bands with lower molecular weights below 22kDa are p17 ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle (AT))
Loading amount 50 µg
Specification Skeletal muscle (AT)
Gel Running Conditions Reduced Denaturing (15% gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. G.K Sakellariou

Verified customer

Submitted Sep 28 2012

Thank you for your reply.

There are a few things I would suggest may help improve the results:

1. I would suggest loading a higher quantity onto your gel, a good starting point would be 20 ug/lane.

2. I would suggest not u...

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Thank you for contacting us.

I do not currenlty have acess to the dilution of secondary antibody used for theWestern blot presented on the datasheet of the Anti-Caspase 3 antibody (ab90437). However, we have a further report of the anitbody u...

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Thank you for completing our survey of your experience with Abcam's Scientific support.

I just wanted to follow up since your replies indicate that the issues you were having with the caspsase 3 antibody, ab90437, were not resolved satisfact...

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"