Caspase 3 Inhibitor Drug Detection Kit (ab102491)

Overview

  • Product name
    Caspase 3 Inhibitor Drug Detection Kit
    See all Caspase 3 kits
  • Detection method
    Fluorescent
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Quantitative
  • Assay time
    1h 30m
  • Product overview

    Abcam's Caspase 3 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate DEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 3 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 3 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab102491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • Titration of the Caspase inhibitor Z-VAD(OMe)-FMK (ab120487) (duplicates; +/- SD).

  • Titration of the Caspase 3 specific inhibitor Q-DEVD-OPh (ab142037) (duplicates; +/- SD).

  • Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using DEVD-AFC as caspase 3 substrate and following Caspase 3 Inhibitor Drug Detection Kit (ab102491) protocol. Lanes:

    1.- Background Control: no Caspase 3, no z-VMAD-FMK

    2.- Positive Inhibition Control: no Caspase 3, +z-VAD-FMK

    3.- Positive Control: + Caspase 3, no z-VAD-FMK

    4.- Caspase 3 + 2.5µM z-VAD-FMK

    5.- Caspase 3 + 10µM z-VAD-FMK

    6.- Caspase 3 + 40µM z-VAD-FMK

Protocols

References

ab102491 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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The lab advices as follows:

Onewould have to add DTT to the 2X Reaction buffer before using it for reconstitution of the Active Cappase-3.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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