Anti-Caspase-3 (phospho S150) antibody (ab59425)

Overview

  • Product nameAnti-Caspase-3 (phospho S150) antibody
    See all Caspase-3 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-3 (phospho S150)
  • SpecificityCaspase-3 (Phospho-Ser150) antibody detects endogenous levels of Caspase-3 only when phosphorylated at serine 150.
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-3.
    Database link: P42574

  • Positive control
    • Human lymph node tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab59425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.
WB 1/500 - 1/1000. Predicted molecular weight: 31 kDa.

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • Tissue specificityHighly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similaritiesBelongs to the peptidase C14A family.
  • Post-translational
    modifications
    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A830040C14Rik antibody
    • Apopain antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Casp3a antibody
    • Caspase 3 antibody
    • Caspase 3, apoptosis-related cysteine peptidase antibody
    • Caspase 3, apoptosis-related cysteine protease antibody
    • Caspase 3, apoptosis-related cysteine protease a antibody
    • Caspase-3 subunit p12 antibody
    • CC3 antibody
    • CPP-32 antibody
    • CPP32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • EC 3.4.22.56 antibody
    • LICE antibody
    • mldy antibody
    • OTTHUMP00000165052 antibody
    • OTTHUMP00000165053 antibody
    • OTTHUMP00000165054 antibody
    • PARP cleavage protease antibody
    • Procaspase3 antibody
    • Protein Yama antibody
    • SCA 1 antibody
    • SCA-1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Anti-Caspase-3 (phospho S150) antibody images

  • ab59425, at a 1/50 dilution, staining paraffin embedded human lymph node tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the phosphopeptide.ab59425, at a 1/50 dilution, staining paraffin embedded human lymph node tissue sections by Immunohistochemistry in the absence (left image) or presence (right image) of the phosphopeptide.
  • All lanes : Anti-Caspase-3 (phospho S150) antibody (ab59425)

    Lane 1 : Jurkat cells, treated with Etoposide (25µM, 60mins)
    Lane 2 : Jurkat cells, treated with Etoposide (25µM, 60mins) with blocking phosphopeptide.


    Predicted band size : 31 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 23 kDa (possible isoform).

References for Anti-Caspase-3 (phospho S150) antibody (ab59425)

ab59425 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Loading amount 30 µg
Specification HCT116
Gel Running Conditions Non-reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Nov 27 2012

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