Anti-Caspase-4 antibody (ab25898)
Key features and details
- Rabbit polyclonal to Caspase-4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Caspase-4 antibody
See all Caspase-4 primary antibodies -
Description
Rabbit polyclonal to Caspase-4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Caspase-4 aa 20-70 (N terminal).
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Positive control
- WB: Ramos cell lysate IHC-P: Mouse spleen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
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IHC-P |
Use a concentration of 2 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa). |
IHC-P
Use a concentration of 2 µg/ml. |
Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. -
Tissue specificity
Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
Post-translational
modificationsThe two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8. - Information by UniProt
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Database links
- Entrez Gene: 837 Human
- Entrez Gene: 12363 Mouse
- Omim: 602664 Human
- SwissProt: P49662 Human
- SwissProt: P70343 Mouse
- Unigene: 138378 Human
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Alternative names
- Apoptotic cysteine protease Mih1/TX antibody
- CASP-4 antibody
- CASP4 antibody
see all
Images
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Lane 1 : Anti-Caspase-4 antibody (ab25898) at 0.5 µg/ml
Lane 2 : Anti-Caspase-4 antibody (ab25898) at 1 µg/ml
All lanes : Ramos cell lysate
Predicted band size: 31 kDa
Observed band size: ~34 kDa why is the actual band size different from the predicted? -
ab25898 Immunohistochemical staining Caspase-4 of mouse spleen using 2 µg/ml (paraffin embedded sections).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab25898 has been referenced in 8 publications.
- Wu Z et al. Double-Edged Sword Effect of Pyroptosis: The Role of Caspase-1/-4/-5/-11 in Different Levels of Apical Periodontitis. Biomolecules 12:N/A (2022). PubMed: 36359010
- Cheng Q et al. Caspase-11/4 and gasdermin D-mediated pyroptosis contributes to podocyte injury in mouse diabetic nephropathy. Acta Pharmacol Sin 42:954-963 (2021). PubMed: 32968210
- Meng J et al. Dendrobine suppresses endoplasmic reticulum stress-induced apoptosis through upregulating microRNA miR-381-3p to decrease caspase-4. Bioengineered 12:4452-4463 (2021). PubMed: 34308746
- Wang X et al. SNX10-mediated LPS sensing causes intestinal barrier dysfunction via a caspase-5-dependent signaling cascade. EMBO J 40:e108080 (2021). PubMed: 34747049
- Kong M et al. miR-512-3p Overcomes Resistance to Cisplatin in Retinoblastoma by Promoting Apoptosis Induced by Endoplasmic Reticulum Stress. Med Sci Monit 26:e923817 (2020). PubMed: 32641679
- Zhang Z et al. Caspase-11-mediated tubular epithelial pyroptosis underlies contrast-induced acute kidney injury. Cell Death Dis 9:983 (2018). PubMed: 30250284
- Ghavami S et al. Apoptosis, autophagy and ER stress in mevalonate cascade inhibition-induced cell death of human atrial fibroblasts. Cell Death Dis 3:e330 (2012). WB ; Human . PubMed: 22717585
- Sansanwal P et al. Caspase-4 may play a role in loss of proximal tubules and renal injury in nephropathic cystinosis. Pediatr Nephrol : (2009). IHC-P ; Human . PubMed: 19705160