Overview

  • Product name
  • Description
    Rabbit polyclonal to Caspase 5
  • Specificity
    This antibody detects a cleaved fragment of activated CASP5 (the p10 subunit), resulting from cleavage adjacent to Ser331.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized peptide derived from human CASP5.

  • Positive control
    • Extracts from 293 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 10 kDa).
ELISA 1/20000.

Target

  • Function
    Mediator of programmed cell death (apoptosis).
  • Tissue specificity
    Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
  • Sequence similarities
    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis related cysteine protease antibody
    • CASP-5 antibody
    • CASP5 antibody
    • CASP5_HUMAN antibody
    • Caspase-5 subunit p10 antibody
    • ICE(rel)-III antibody
    • ICERELIII antibody
    • ICH 3 antibody
    • ICH 3 protease antibody
    • Protease ICH-3 antibody
    • Protease TY antibody
    • TY antibody
    • TY protease antibody
    see all

Anti-Caspase 5 antibody images

  • All lanes : Anti-Caspase 5 antibody (ab69637) at 1/500 dilution

    Lane 1 : Extracts from 293 cells treated with etoposide (25uM, 1hour)
    Lane 2 : Extracts from 293 cells treated with etoposide (25uM, 1hour), with immunising cleavage peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 10 kDa
    Observed band size : <19 kDa (why is the actual band size different from the predicted?)

References for Anti-Caspase 5 antibody (ab69637)

ab69637 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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