Overview

  • Product nameAnti-Caspase 5 antibody
    See all Caspase 5 primary antibodies
  • Description
    Rabbit polyclonal to Caspase 5
  • SpecificityThis antibody detects a cleaved fragment of activated CASP5 (the p10 subunit), resulting from cleavage adjacent to Ser331.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized peptide derived from human CASP5.

  • Positive control
    • Extracts from 293 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 10 kDa).
ELISA 1/20000.

Target

  • FunctionMediator of programmed cell death (apoptosis).
  • Tissue specificityExpressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Post-translational
    modifications
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis related cysteine protease antibody
    • CASP-5 antibody
    • CASP5 antibody
    • CASP5_HUMAN antibody
    • Caspase-5 subunit p10 antibody
    • ICE(rel)-III antibody
    • ICERELIII antibody
    • ICH 3 antibody
    • ICH 3 protease antibody
    • Protease ICH-3 antibody
    • Protease TY antibody
    • TY antibody
    • TY protease antibody
    see all

Anti-Caspase 5 antibody images

  • All lanes : Anti-Caspase 5 antibody (ab69637) at 1/500 dilution

    Lane 1 : Extracts from 293 cells treated with etoposide (25uM, 1hour)
    Lane 2 : Extracts from 293 cells treated with etoposide (25uM, 1hour), with immunising cleavage peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 10 kDa
    Observed band size : <19 kDa (why is the actual band size different from the predicted?)

References for Anti-Caspase 5 antibody (ab69637)

ab69637 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab69637.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"