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Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes ELISA: 1/20000.
WB: 1/500 - 1/1000. Detects a band of approximately 26 and 48 kDa (predicted molecular weight of precursor): 48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Function Mediator of programmed cell death (apoptosis).
Tissue specificity Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
Sequence similarities Belongs to the peptidase C14A family. Contains 1 CARD domain.
Post-translational modifications The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
Information by UniProt
Apoptosis related cysteine protease antibody
Anti-Caspase 5 antibody images
Western blot - Caspase 5 antibody (ab69641)
All lanes : Anti-Caspase 5 antibody (ab69641) at 1/500 dilution Lane 1 : HeLa cells treated with etoposide (25uM, 24hours) Lane 2 :
A549 cells treated with etoposide (25uM, 24hours) Lane 3 : HeLa cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 48 kDa Observed band size : 26,48 kDa ( why is the actual band size different from the predicted?) Additional bands at : 26 kDa (possible cleavage fragment).
References for Anti-Caspase 5 antibody (ab69641)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"