Overview

  • Product name
  • Description
    Rabbit polyclonal to Caspase 5
  • Specificity
    ab69641 can detect a fragment of activated Caspase 5 resulting from cleavage adjacent to Asp121.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthesized peptide derived from human Caspase 5

  • Positive control
    • Extracts from HeLa cells and A549 cells, treated with etoposide (25uM, 24hours).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/20000.
    WB: 1/500 - 1/1000. Detects a band of approximately 26 and 48 kDa (predicted molecular weight of precursor): 48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Mediator of programmed cell death (apoptosis).
    • Tissue specificity
      Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
    • Sequence similarities
      Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • Post-translational
      modifications
      The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptosis related cysteine protease antibody
      • CASP-5 antibody
      • CASP5 antibody
      • CASP5_HUMAN antibody
      • Caspase-5 subunit p10 antibody
      • ICE(rel)-III antibody
      • ICERELIII antibody
      • ICH 3 antibody
      • ICH 3 protease antibody
      • Protease ICH-3 antibody
      • Protease TY antibody
      • TY antibody
      • TY protease antibody
      see all

    Images

    • All lanes : Anti-Caspase 5 antibody (ab69641) at 1/500 dilution

      Lane 1 : HeLa cells treated with etoposide (25uM, 24hours)
      Lane 2 : A549 cells treated with etoposide (25uM, 24hours)
      Lane 3 : HeLa cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 48 kDa
      Observed band size : 26,48 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 26 kDa (possible cleavage fragment).

    References

    ab69641 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab69641.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up