Overview

  • Product nameAnti-Caspase 5 antibody
    See all Caspase 5 primary antibodies
  • Description
    Rabbit polyclonal to Caspase 5
  • Specificityab69641 can detect a fragment of activated Caspase 5 resulting from cleavage adjacent to Asp121.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthesized peptide derived from human Caspase 5

  • Positive control
    • Extracts from HeLa cells and A549 cells, treated with etoposide (25uM, 24hours).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/20000.
    WB: 1/500 - 1/1000. Detects a band of approximately 26 and 48 kDa (predicted molecular weight of precursor): 48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMediator of programmed cell death (apoptosis).
    • Tissue specificityExpressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
    • Sequence similaritiesBelongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • Post-translational
      modifications
      The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptosis related cysteine protease antibody
      • CASP-5 antibody
      • CASP5 antibody
      • CASP5_HUMAN antibody
      • Caspase-5 subunit p10 antibody
      • ICE(rel)-III antibody
      • ICERELIII antibody
      • ICH 3 antibody
      • ICH 3 protease antibody
      • Protease ICH-3 antibody
      • Protease TY antibody
      • TY antibody
      • TY protease antibody
      see all

    Anti-Caspase 5 antibody images

    • All lanes : Anti-Caspase 5 antibody (ab69641) at 1/500 dilution

      Lane 1 : HeLa cells treated with etoposide (25uM, 24hours)
      Lane 2 : A549 cells treated with etoposide (25uM, 24hours)
      Lane 3 : HeLa cells treated with etoposide (25uM, 24hours) with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 48 kDa
      Observed band size : 26,48 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 26 kDa (possible cleavage fragment).

    References for Anti-Caspase 5 antibody (ab69641)

    ab69641 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab69641.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"