Anti-Caspase 5 antibody [CAS5] (ab10448)

Overview

  • Product nameAnti-Caspase 5 antibody [CAS5]
    See all Caspase 5 primary antibodies
  • Description
    Mouse monoclonal [CAS5] to Caspase 5
  • Tested applicationsSuitable for: ELISA, WB, Double Immunodiffusionmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    GEKHGELWVR

    conjugated to KLH, corresponding to amino acids 300-311 of Human Caspase 5.

  • EpitopeThe epitope recognized by the antibody resides within amino acids 300-311 of human Caspase 5 molecule.
  • Positive control
    • Whole extract of HeLa cells activated with lipopolysaccharide (LPS).
  • General notesFor continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberCAS5
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
Double Immunodiffusion
  • Application notesDID: Use at an assay dependent dilution.
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 5 - 10 µg/ml (determined by using a whole extract of HeLa cells activated with lipopolysaccharide (LPS)). Expected bands: a doublet at approx. 42 kDa, with additional bands at approx. 75 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMediator of programmed cell death (apoptosis).
    • Tissue specificityExpressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
    • Sequence similaritiesBelongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • Post-translational
      modifications
      The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptosis related cysteine protease antibody
      • CASP-5 antibody
      • CASP5 antibody
      • CASP5_HUMAN antibody
      • Caspase-5 subunit p10 antibody
      • ICE(rel)-III antibody
      • ICERELIII antibody
      • ICH 3 antibody
      • ICH 3 protease antibody
      • Protease ICH-3 antibody
      • Protease TY antibody
      • TY antibody
      • TY protease antibody
      see all

    References for Anti-Caspase 5 antibody [CAS5] (ab10448)

    ab10448 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for the information that you have provided from your end-user. I'm sorry that the antibody did not work out for the researcher. I have contacted our accounting department and a refund will be issued for your order for ab10448. If you have any...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"