Recombinant
RabMAb

Anti-Caspase 5 antibody [EP876Y] (ab40887)

Overview

  • Product name
    Anti-Caspase 5 antibody [EP876Y]
    See all Caspase 5 primary antibodies
  • Description
    Rabbit monoclonal [EP876Y] to Caspase 5
  • Specificity
    Based on amino acids homology, this antibody will not recognize either p20 or p10 subunits of Caspase-5.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IP, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of human Caspase-5

  • Positive control
    • HeLa cells, human breast carcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EP876Y
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
IP 1/50.
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Mediator of programmed cell death (apoptosis).
    • Tissue specificity
      Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.
    • Sequence similarities
      Belongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • Post-translational
      modifications
      The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptosis related cysteine protease antibody
      • CASP-5 antibody
      • CASP5 antibody
      • CASP5_HUMAN antibody
      • Caspase-5 subunit p10 antibody
      • ICE(rel)-III antibody
      • ICERELIII antibody
      • ICH 3 antibody
      • ICH 3 protease antibody
      • Protease ICH-3 antibody
      • Protease TY antibody
      • TY antibody
      • TY protease antibody
      see all

    Images

    • Anti-Caspase 5 antibody [EP876Y] (ab40887) at 1/10000 dilution + HeLa cell lysate at 10 µg

      Predicted band size : 47 kDa
      Observed band size : 47 kDa
    • Stainign of Caspase 5 on paraffin-embedded human breast carcinoma sections, using ab40557 at a 1:50 dilution.
    • ab40887 staining HeLa cells for Caspase 5 (dilution 1:100).

    References

    This product has been referenced in:
    • Manchado E  et al. Targeting Mitotic Exit Leads to Tumor Regression In Vivo: Modulation by Cdk1, Mastl, and the PP2A/B55a,d Phosphatase. Cancer Cell 18:641-54 (2010). WB ; Mouse . Read more (PubMed: 21156286) »
    • Williams DS  et al. Nonsense mediated decay resistant mutations are a source of expressed mutant proteins in colon cancer cell lines with microsatellite instability. PLoS One 5:e16012 (2010). Read more (PubMed: 21209843) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry. I have contacted the originators of these antibodies and here is the sequence information I obtained. ab25899: This immunogen sequence is proprietary, but we can run any alignments with the sequence that you require. ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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