The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 34 kDa).
Use a concentration of 20 µg/ml.
Use a concentration of 1 µg/ml.
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
Sequence similaritiesBelongs to the peptidase C14A family.
Post-translational modificationsCleavages by caspase-3, caspase-8 or -10 generate the two active subunits.