Overview

  • Product nameAnti-Caspase-6 antibody
    See all Caspase-6 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-6
  • Specificityab63487 detects endogenous levels of total Caspase-6 protein.
  • Tested applicationsSuitable for: ELISA, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-6. Immunogen is within aa from 223-272.
    Database link: P55212

  • Positive control
    • IHC-P: Human breast carcinoma tissue ICC/IF: HuvEc cells WB: A431 cell extracts

Properties

Applications

Our Abpromise guarantee covers the use of ab63487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 33 kDa.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
  • Sequence similaritiesBelongs to the peptidase C14A family.
  • Post-translational
    modifications
    Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptotic protease Mch-2 antibody
    • Apoptotic protease MCH2 antibody
    • CASP-6 antibody
    • CASP6 antibody
    • CASP6_HUMAN antibody
    • Caspase 6 antibody
    • Caspase 6 apoptosis related cysteine protease antibody
    • Caspase 6, apoptosis related cysteine peptidase antibody
    • Caspase-6 subunit p11 antibody
    • Mch2 antibody
    • OTTHUMP00000162742 antibody
    • OTTHUMP00000162743 antibody
    see all

Anti-Caspase-6 antibody images

  • ab63487, at 1/500 dilution, staining Caspase-6 in HuvEc cells by Immunofluorescence, in the absence (left image) or presence (right image) of the immunising peptide.

  • ab63487, at 1:50 dilution, staining Caspase-6 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.

  • All lanes : Anti-Caspase-6 antibody (ab63487) at 1/500 dilution

    Lane 1 : A431 cell extracts
    Lane 2 : A431 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 33 kDa
    Additional bands at : 25 kDa,30 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Caspase-6 antibody (ab63487)

ab63487 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"