Anti-Caspase-6 antibody [E180] (ab32366)


  • Product nameAnti-Caspase-6 antibody [E180]
    See all Caspase-6 primary antibodies
  • Description
    Rabbit monoclonal [E180] to Caspase-6
  • SpecificityThis antibody detects the pro form and the p11 subunit of human Caspase-6. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-6 aa 250-350 (C terminal).
    Database link: P55212

  • Positive control
    • WB: Jurkat cell lysate. ICC/IF: Hela cells. IHC-P: Human lung carcinoma tissue slides.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.


Associated products


Our Abpromise guarantee covers the use of ab32366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
    • Sequence similaritiesBelongs to the peptidase C14A family.
    • Post-translational
      Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptotic protease Mch-2 antibody
      • Apoptotic protease MCH2 antibody
      • CASP-6 antibody
      • CASP6 antibody
      • CASP6_HUMAN antibody
      • Caspase 6 antibody
      • Caspase 6 apoptosis related cysteine protease antibody
      • Caspase 6, apoptosis related cysteine peptidase antibody
      • Caspase-6 subunit p11 antibody
      • Mch2 antibody
      • OTTHUMP00000162742 antibody
      • OTTHUMP00000162743 antibody
      see all

    Anti-Caspase-6 antibody [E180] images

    • Ab32366, at a 1:50 dilution, staining Caspase-6 in paraffin embedded human lung adenocarcinoma tissue by Immunohistochemistry.

    • Ab32366, at a 1:100 dilution, staining Caspase-6 in Hela cells by Immunocytochemistry/Immunofluorescence.

    • All lanes : Anti-Caspase-6 antibody [E180] (ab32366) at 1/1000 dilution

      Lane 1 : Untreated Jurkat cell lysate.
      Lane 2 : Jurkat cell lysate treated with Camptothecin.

      Predicted band size : 34 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    References for Anti-Caspase-6 antibody [E180] (ab32366)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Tonsil)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Specification Tonsil
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative Formaldehyde

    Mr. Carl Hobbs

    Verified customer

    Submitted Feb 13 2017