Recombinant
RabMAb

Anti-Caspase-6 antibody [E180] (ab32366)

Overview

  • Product name
    Anti-Caspase-6 antibody [E180]
    See all Caspase-6 primary antibodies
  • Description
    Rabbit monoclonal [E180] to Caspase-6
  • Specificity
    This antibody detects the pro form and the p11 subunit of human Caspase-6. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-6 aa 250-350 (C terminal).
    Database link: P55212

  • Positive control
    • WB: Jurkat cell lysate. ICC/IF: Hela cells. IHC-P: Human lung carcinoma tissue slides.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
    • Sequence similarities
      Belongs to the peptidase C14A family.
    • Post-translational
      modifications
      Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptotic protease Mch-2 antibody
      • Apoptotic protease MCH2 antibody
      • CASP-6 antibody
      • CASP6 antibody
      • CASP6_HUMAN antibody
      • Caspase 6 antibody
      • Caspase 6 apoptosis related cysteine protease antibody
      • Caspase 6, apoptosis related cysteine peptidase antibody
      • Caspase-6 subunit p11 antibody
      • Mch2 antibody
      • OTTHUMP00000162742 antibody
      • OTTHUMP00000162743 antibody
      see all

    Images

    • Ab32366, at a 1:50 dilution, staining Caspase-6 in paraffin embedded human lung adenocarcinoma tissue by Immunohistochemistry.

    • Ab32366, at a 1:100 dilution, staining Caspase-6 in Hela cells by Immunocytochemistry/Immunofluorescence.

    • All lanes : Anti-Caspase-6 antibody [E180] (ab32366) at 1/1000 dilution

      Lane 1 : Untreated Jurkat cell lysate.
      Lane 2 : Jurkat cell lysate treated with Camptothecin.


      Predicted band size : 34 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Feb 13 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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