Recombinant Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E180] to Caspase-6/CASP-6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Caspase-6/CASP-6 antibody [E180]
See all Caspase-6/CASP-6 primary antibodies -
Description
Rabbit monoclonal [E180] to Caspase-6/CASP-6 -
Host species
Rabbit -
Specificity
This antibody detects the pro form and the p11 subunit of human Caspase-6/CASP-6. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
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Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human Caspase-6/CASP-6 aa 250-350 (C terminal). The exact sequence is proprietary.
Database link: P55212 -
Positive control
- WB: Jurkat cell lysate. ICC/IF: Hela cells. IHC-P: Human lung carcinoma tissue slides.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E180 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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Notes |
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WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Target
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Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. -
Sequence similarities
Belongs to the peptidase C14A family. -
Post-translational
modificationsCleavages by caspase-3, caspase-8 or -10 generate the two active subunits. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 839 Human
- Entrez Gene: 12368 Mouse
- Omim: 601532 Human
- SwissProt: P55212 Human
- SwissProt: O08738 Mouse
- Unigene: 654616 Human
- Unigene: 281379 Mouse
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Alternative names
- Apoptotic protease Mch-2 antibody
- Apoptotic protease MCH2 antibody
- CASP-6 antibody
see all
Images
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All lanes : Anti-Caspase-6/CASP-6 antibody [E180] (ab32366) at 1/1000 dilution
Lane 1 : Untreated Jurkat cell lysate.
Lane 2 : Jurkat cell lysate treated with Camptothecin.
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Ab32366, at a 1:50 dilution, staining Caspase-6/CASP-6 in paraffin embedded human lung adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Ab32366, at a 1:100 dilution, staining Caspase-6/CASP-6 in Hela cells by Immunocytochemistry/Immunofluorescence.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab32366 has been referenced in 1 publication.
- Coutinho-Camillo CM et al. Caspase expression in oral squamous cell carcinoma. Head Neck 33:1191-8 (2011). IHC ; Human . PubMed: 21755562