Anti-Caspase-6 antibody [EPR4405] (ab108335)

Overview

  • Product nameAnti-Caspase-6 antibody [EPR4405]
    See all Caspase-6 primary antibodies
  • Description
    Rabbit monoclonal [EPR4405] to Caspase-6
  • Specificityab108335 detects both the full length and cleaved forms of Caspase-6.
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caspase-6 aa 150-250.
    Database link: P55212

  • Positive control
    • Jurkat cells and cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
    • Sequence similaritiesBelongs to the peptidase C14A family.
    • Post-translational
      modifications
      Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptotic protease Mch-2 antibody
      • Apoptotic protease MCH2 antibody
      • CASP-6 antibody
      • CASP6 antibody
      • CASP6_HUMAN antibody
      • Caspase 6 antibody
      • Caspase 6 apoptosis related cysteine protease antibody
      • Caspase 6, apoptosis related cysteine peptidase antibody
      • Caspase-6 subunit p11 antibody
      • Mch2 antibody
      • OTTHUMP00000162742 antibody
      • OTTHUMP00000162743 antibody
      see all

    Anti-Caspase-6 antibody [EPR4405] images

    • All lanes : Anti-Caspase-6 antibody [EPR4405] (ab108335) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Jurkat + staurosporine cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 33 kDa
    • ab108335, at 1:10 dilution, staining Caspase-6 in permeabilized Jurkat cells by Flow cytometry (shown in red). Rabbit IgG shown in green (negative).

    References for Anti-Caspase-6 antibody [EPR4405] (ab108335)

    ab108335 has not yet been referenced specifically in any publications.

    Product Wall

    Application Flow Cytometry
    Fixation Formaldehyde
    Permeabilization Yes - 0.1% PBS-Tween
    Sample Mouse Cell (ledyig cells)
    Specification ledyig cells
    Gating Strategy RLF positive cells
    Preparation Cell harvesting/tissue preparation method: Tubules from testes were incubated for 15–20 min at 35¯C in a shaking water bath at 80 oscillations (osc)/min , then layered over 40 mL 5% Percoll and leydig cells were isolated from top of the percoll
    Sample buffer: 0.1% PBS BSA
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    Submitted Dec 04 2013

    Application Flow Cytometry
    Fixation Methanol
    Permeabilization Yes - 0.1% PBS-Tween
    Sample Rat Cell (breast cells)
    Specification breast cells
    Gating Strategy cd1d1 POSITIVE
    Preparation Cell harvesting/tissue preparation method: tissue was treated with collagenase to make single cell suspension
    Sample buffer: 0.1% PBS BSA
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    Submitted Dec 04 2013

    Application Flow Cytometry
    Fixation Methanol
    Permeabilization Yes - 0.1% PBS-Tween
    Sample Human Cell (MCF7)
    Specification MCF7
    Gating Strategy CD44 POSITIVE
    Preparation Cell harvesting/tissue preparation method: treated with collagenase and trypsin to make single cell suspension
    Sample buffer: 0.1% PBS BSA
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    Submitted Dec 04 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Immuno-precipitation step Staph. aureus
    Sample Human Cell lysate - whole cell (MCF7)
    Specification MCF7
    Total protein in input 100 µg
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    Submitted Sep 09 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (10)
    Sample Human Cell lysate - whole cell (MCF7)
    Specification MCF7
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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    Submitted Sep 06 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Immuno-precipitation step Staph. aureus
    Sample Human Cell lysate - whole cell (mcf7)
    Specification mcf7
    Total protein in input 50 µg
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    Submitted Jun 27 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"