Caspase 6 Inhibitor Drug Detection Kit (ab102494)

Overview

  • Product nameCaspase 6 Inhibitor Drug Detection Kit
    See all Caspase 6 kits
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Assay time
    1h 30m
  • Product overview

    Abcam's Caspase 6 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate VEID-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 6 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 6 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

  • Tested applicationsSuitable for: Functional Studiesmore details

Properties

  • RelevanceCaspase 6 is involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Caspase 3 and Caspase 6 are the major active caspases in apoptotic cells, and are activated in response to distinct apoptosis inducing stimuli and in all cell lines analyzed. Both Caspase 3 and 6 are present in apoptotic cells as multiple active species. Caspase 6 cleaves nuclear mitotic apparatus protein (NuMA) and mediates the shrinkage and fragmentation of nuclei.
  • Cellular localizationCytoplasmic
  • Alternative names
    • Apoptotic Protease MCH2
    • CASP6
    • Caspase 6 apoptosis related cysteine protease
    • Caspase 6 precursor
    • MCH2
    see all

Associated products

Applications

Our Abpromise guarantee covers the use of ab102494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Protocols

References for Caspase 6 Inhibitor Drug Detection Kit (ab102494)

ab102494 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry. This kit is generally used with cell lysates, but theoretically it could be used for blood and other samples. This kit is intended for research use only because it is not validated for patient clinical samples. It is ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"