Recombinant
RabMAb

Anti-Caspase-6 p18 antibody [EPRR18045] (ab185648)

Overview

  • Product name
    Anti-Caspase-6 p18 antibody [EPRR18045]
  • Description
    Rabbit monoclonal [EPRR18045] to Caspase-6 p18
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human Caspase-6 p18 aa 1-200. The exact sequence is proprietary.
    Database link: P55212

  • Positive control
    • WB: Untreated and treated with 1uM staurosporine for 4 hours Jurkat whole cell lysates. IP: Jurkat treated with 1uM staurosporine for 4 hours whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab185648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 33,18 kDa (predicted molecular weight: 33 kDa).
IP 1/50.

Target

  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
  • Sequence similarities
    Belongs to the peptidase C14A family.
  • Post-translational
    modifications
    Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptotic protease Mch-2 antibody
    • Apoptotic protease MCH2 antibody
    • CASP-6 antibody
    • CASP6 antibody
    • CASP6_HUMAN antibody
    • Caspase 6 antibody
    • Caspase 6 apoptosis related cysteine protease antibody
    • Caspase 6, apoptosis related cysteine peptidase antibody
    • Caspase-6 subunit p11 antibody
    • Caspase-6 subunit p20 antibody
    • Mch2 antibody
    • OTTHUMP00000162742 antibody
    • OTTHUMP00000162743 antibody
    see all

Images

  • All lanes : Anti-Caspase-6 p18 antibody [EPRR18045] (ab185648) at 1/2000 dilution

    Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) treated with 1uM staurosporine
    for 4 hours whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 33 kDa
    Observed band size: 18,33 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Caspase-6 p18 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cell line from peripheral blood) treated with 1uM staurosporine for 4 hours whole cell lysate with ab185648 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab185648 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Jurkat treated with 1uM staurosporine for 4 hours whole cell lysate, 10ug (Input).

    Lane 2: ab185648 IP in Jurkat treated with 1uM staurosporine for 4 hours whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal -  (ab172730) instead of ab185648 in Jurkat treated with 1uM staurosporine for 4 hours whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

References

ab185648 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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