Purification notesab63440 was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
1/500 - 1/1000.
FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
Sequence similaritiesBelongs to the peptidase C14A family.
Post-translational modificationsCleavages by caspase-3, caspase-8 or -10 generate the two active subunits.