Anti-Caspase-6 (phospho S257) antibody (ab63440)

Overview

  • Product nameAnti-Caspase-6 (phospho S257) antibody
    See all Caspase-6 primary antibodies
  • Description
    Rabbit polyclonal to Caspase-6 (phospho S257)
  • Specificityab63440 detects endogenous levels of Caspase-6 only when phosphorylated at serine 257.
  • Tested applicationsSuitable for: ELISA, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-6.
    Database link: P55212

  • Positive control
    • Human colon carcinoma tissue, HuvEc cells and 293 cell extracts treated with Etoposide (25uM, 60min).

Properties

Applications

Our Abpromise guarantee covers the use of ab63440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
ICC/IF 1/500 - 1/1000.

Target

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
  • Sequence similaritiesBelongs to the peptidase C14A family.
  • Post-translational
    modifications
    Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptotic protease Mch-2 antibody
    • Apoptotic protease MCH2 antibody
    • CASP-6 antibody
    • CASP6 antibody
    • CASP6_HUMAN antibody
    • Caspase 6 antibody
    • Caspase 6 apoptosis related cysteine protease antibody
    • Caspase 6, apoptosis related cysteine peptidase antibody
    • Caspase-6 subunit p11 antibody
    • Mch2 antibody
    • OTTHUMP00000162742 antibody
    • OTTHUMP00000162743 antibody
    see all

Anti-Caspase-6 (phospho S257) antibody images

  • ab63440 at 1:500 - 1:1000 dilution staining Caspase-6 in HuvEc cells by Immunofluorescence, in the absence or presence of the immunising peptide.

  • ab63440 at 1:50 - 1:100 dilution staining Caspase-6 in human colon carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

  • All lanes : Anti-Caspase-6 (phospho S257) antibody (ab63440) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with Etoposide (25uM, 60min)
    Lane 2 : 293 cell extracts treated with Etoposide (25uM, 60min) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    The amount of positive control loading is generally 5-30 ug of total protein. The amount of the peptide is 5-10 ug.

References for Anti-Caspase-6 (phospho S257) antibody (ab63440)

ab63440 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"