Overview

  • Product name
    Anti-Caspase-7 antibody [10-1-62]
    See all Caspase-7 primary antibodies
  • Description
    Mouse monoclonal [10-1-62] to Caspase-7
  • Specificity
    Ab28130 recognises Caspase-7, recombinant caspase-7 as well as full length pro caspase-7 and processed enzyme.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human Caspase-7.
    Database link: P55210

  • Positive control
    • HeLa and Jurkat cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab28130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.

Target

  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Overexpression promotes programmed cell death.
  • Tissue specificity
    Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
  • Sequence similarities
    Belongs to the peptidase C14A family.
  • Post-translational
    modifications
    Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptotic protease Mch-3 antibody
    • Apoptotic protease MCH3 antibody
    • CASP-7 antibody
    • CASP7 antibody
    • CASP7_HUMAN antibody
    • Caspase 7 antibody
    • Caspase 7 apoptosis related cysteine peptidase antibody
    • Caspase-7 subunit p11 antibody
    • Caspase7 antibody
    • CMH 1 antibody
    • CMH-1 antibody
    • CMH1 antibody
    • ICE LAP3 antibody
    • ICE-LAP3 antibody
    • ICE-like apoptotic protease 3 antibody
    • LICE2 antibody
    • MCH3 antibody
    see all

Images

  • Immunohistochemistry paraffin embedded sections analysis of Human colon tissue sections labeling Caspase-7 with ab28130 at 10µg/ml.



  • Predicted band size : 34 kDa

    Western Blot analysis of human Jurkat and Jurkat induced cell lysates, probed with Caspase-7 antibody (ab28130).

  • Immunohistochemistry paraffin embedded sections analysis of Human spleen tissue sections labeling Caspase-7 with ab28130 at 10µg/ml.

References

This product has been referenced in:
  • Feng H  et al. Evidence for a Novel, Caspase-8-Independent, Fas Death Domain-Mediated Apoptotic Pathway. J Biomed Biotechnol 2004:41-51 (2004). Read more (PubMed: 15123887) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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