Overview

  • Product name
  • Description
    Rabbit polyclonal to Caspase-7
  • Specificity
    ab25900 recognises Caspase-7.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-7 aa 1-16 (C terminal).
    Database link: P55210

  • Positive control
    • Jurkat cell lysate, human skeletal muscle cell

Properties

Applications

Our Abpromise guarantee covers the use of ab25900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).

Target

  • Function
    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Overexpression promotes programmed cell death.
  • Tissue specificity
    Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.
  • Sequence similarities
    Belongs to the peptidase C14A family.
  • Post-translational
    modifications
    Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptotic protease Mch-3 antibody
    • Apoptotic protease MCH3 antibody
    • CASP-7 antibody
    • CASP7 antibody
    • CASP7_HUMAN antibody
    • Caspase 7 antibody
    • Caspase 7 apoptosis related cysteine peptidase antibody
    • Caspase-7 subunit p11 antibody
    • Caspase7 antibody
    • CMH 1 antibody
    • CMH-1 antibody
    • CMH1 antibody
    • ICE LAP3 antibody
    • ICE-LAP3 antibody
    • ICE-like apoptotic protease 3 antibody
    • LICE2 antibody
    • MCH3 antibody
    see all

Images

  • Lane 1 : Anti-Caspase-7 antibody (ab25900) at 0.5 µg/ml
    Lane 2 : Anti-Caspase-7 antibody (ab25900) at 1 µg/ml
    Lane 3 : Anti-Caspase-7 antibody (ab25900) at 2 µg/ml

    Lane 1 : Human skeletal muscle cell lysate
    Lane 2 : Human skeletal muscle cell lysate
    Lane 3 : Human skeletal muscle cell lysate


    Predicted band size : 34 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical staining of human skeletal muscle (paraffin embedded sections) using ab25900 anti Caspase-7 at 2 µg/ml.

References

This product has been referenced in:
  • Ma J  et al. Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles. Cell Res 26:713-27 (2016). Read more (PubMed: 27167569) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (NSCLC lung cancer cell)
Loading amount
50 µg
Specification
NSCLC lung cancer cell
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 14 2011

Thank you for your enquiry. With regards to specificity of this antiserum we are satisfied that it recognizes the murine homolog of the epitope, albeit as determined by western blotting. Unfortunately on this occasion I cannot offer you a free ...

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Thank you for your enquiry. Further to correspondence with the source of this antiserum, I have determined that with respect to murine cells, ab25900 has only been tested by western blotting. IHC testing at this time has only been performed on paraf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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